A Ni-Fe-Cu center in a bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase

Tzanko I. Doukov, Tina M. Iverson, Javier Seravalli, Stephen W. Ragsdale, Catherine L. Drennan

Research output: Contribution to journalArticle

370 Citations (Scopus)

Abstract

A metallocofactor containing iron, sulfur, copper, and nickel has been discovered in the enzyme carbon monoxide dehydrogenase/acetyl-CoA (coenzyme A) synthase from Moorella thermoacetica (f. Clostridium thermoaceticum). Our structure at 2.2 angstrom resolution reveals that the cofactor responsible for the assembly of acetyl-CoA contains a [Fe4S4] cubane bridged to a copper-nickel binuclear site. The presence of these three metals together in one cluster was unanticipated and suggests a newly discovered role for copper in biology. The different active sites of this bifunctional enzyme complex are connected via a channel, 138 angstroms long, that provides a conduit for carbon monoxide generated at the C-cluster on one subunit to be incorporated into acetyl-CoA at the A-cluster on the other subunit.

Original languageEnglish (US)
Pages (from-to)567-572
Number of pages6
JournalScience
Volume298
Issue number5593
DOIs
StatePublished - Oct 18 2002

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carbon monoxide dehydrogenase
Acetyl Coenzyme A
Copper
Nickel
Moorella
Clostridium
Enzymes
Coenzyme A
Carbon Monoxide
Sulfur
Catalytic Domain
Iron
Metals

ASJC Scopus subject areas

  • General

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A Ni-Fe-Cu center in a bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase. / Doukov, Tzanko I.; Iverson, Tina M.; Seravalli, Javier; Ragsdale, Stephen W.; Drennan, Catherine L.

In: Science, Vol. 298, No. 5593, 18.10.2002, p. 567-572.

Research output: Contribution to journalArticle

Doukov, TI, Iverson, TM, Seravalli, J, Ragsdale, SW & Drennan, CL 2002, 'A Ni-Fe-Cu center in a bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase', Science, vol. 298, no. 5593, pp. 567-572. https://doi.org/10.1126/science.1075843
Doukov, Tzanko I. ; Iverson, Tina M. ; Seravalli, Javier ; Ragsdale, Stephen W. ; Drennan, Catherine L. / A Ni-Fe-Cu center in a bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase. In: Science. 2002 ; Vol. 298, No. 5593. pp. 567-572.
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