A newly established metastatic breast tumor cell line with integrated amplified copies of ERBB2 and double minute chromosomes

Vimla Band, Deborah Zajchowski, Göran Stenman, Cynthia C. Morton, Victoria Kulesa, James Connolly, Ruth Sager

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A continuous line of human mammary tumor cells, called 21MT, has been established in culture from a pleural effusion of a 36‐year‐old woman with metastatic breast cancer. The cells are epithelial as shown by morphology and expression of keratins and are mammary tumor cells as shown by expression of the HMFG‐2 antigenic determinant. The cells grow well both in DFCI‐1, a partially defined medium containing pituitary extract and 1% fetal bovine serum, and in α‐minimum essential medium (α‐MEM) supplemented with 10% serum, epidermal growth factor (EGF), insulin, and hydrocortisone. Karyotypic analysis of cells at early passage has shown the presence of rearranged (marker) chromosomes as well as aneuploidy with a net DNA content in the tetraploid range, confirmed by DNA cytofluorography, as well as double minute chromosomes in about 5% of the cells. Southern blots have revealed a 40‐fold amplification of the ERBB2 gene and a 50‐fold overexpression of its mRNA. The amplification of ERBB2 DNA was localized by in situ hybridization to one of the marker chromosomes but not to the double minutes. It is inferred, therefore, that at least two genes have been amplified in these cells.

Original languageEnglish (US)
Pages (from-to)48-58
Number of pages11
JournalGenes, Chromosomes and Cancer
Volume1
Issue number1
DOIs
StatePublished - Jan 1 1989

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Tumor Cell Line
Chromosomes
Breast Neoplasms
Genetic Markers
DNA
Tetraploidy
Gene Amplification
Aneuploidy
Pleural Effusion
Keratins
Southern Blotting
Serum
Epidermal Growth Factor
In Situ Hybridization
Hydrocortisone
Epitopes
Epithelial Cells
Insulin
Messenger RNA
Genes

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

Cite this

A newly established metastatic breast tumor cell line with integrated amplified copies of ERBB2 and double minute chromosomes. / Band, Vimla; Zajchowski, Deborah; Stenman, Göran; Morton, Cynthia C.; Kulesa, Victoria; Connolly, James; Sager, Ruth.

In: Genes, Chromosomes and Cancer, Vol. 1, No. 1, 01.01.1989, p. 48-58.

Research output: Contribution to journalArticle

Band, Vimla ; Zajchowski, Deborah ; Stenman, Göran ; Morton, Cynthia C. ; Kulesa, Victoria ; Connolly, James ; Sager, Ruth. / A newly established metastatic breast tumor cell line with integrated amplified copies of ERBB2 and double minute chromosomes. In: Genes, Chromosomes and Cancer. 1989 ; Vol. 1, No. 1. pp. 48-58.
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abstract = "A continuous line of human mammary tumor cells, called 21MT, has been established in culture from a pleural effusion of a 36‐year‐old woman with metastatic breast cancer. The cells are epithelial as shown by morphology and expression of keratins and are mammary tumor cells as shown by expression of the HMFG‐2 antigenic determinant. The cells grow well both in DFCI‐1, a partially defined medium containing pituitary extract and 1{\%} fetal bovine serum, and in α‐minimum essential medium (α‐MEM) supplemented with 10{\%} serum, epidermal growth factor (EGF), insulin, and hydrocortisone. Karyotypic analysis of cells at early passage has shown the presence of rearranged (marker) chromosomes as well as aneuploidy with a net DNA content in the tetraploid range, confirmed by DNA cytofluorography, as well as double minute chromosomes in about 5{\%} of the cells. Southern blots have revealed a 40‐fold amplification of the ERBB2 gene and a 50‐fold overexpression of its mRNA. The amplification of ERBB2 DNA was localized by in situ hybridization to one of the marker chromosomes but not to the double minutes. It is inferred, therefore, that at least two genes have been amplified in these cells.",
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