A new class of antivirals: antisense oligonucleotides combined with a hydrophobic substituent effectively inhibit influenza virus reproduction and synthesis of virus-specific proteins in MDCK cells

Alexander V. Kabanov, Sergei V. Vinogradov, Alexander V. Ovcharenko, Alexander V. Krivonos, Nikolai S. Melik-Nubarov, Vsevolod I. Kiselev, Eugenii S. Severin

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

To enhance the penetration of oligonucleotide ('oligo') into cells, the oligo was combined with the hydrophobic undecyl residue. Using the 'DNA-synthesator', we synthesized oligo, complementary to the loop-forming site of the RNA, encoding polymerase 3 of the influenza virus (type A), and combined it with the undecyl residue added to the 5' terminal phosphate group. It was found that the modified oligo effectively suppresses the influenza A/PR8/34 (H1N1) virus reproduction and inhibits the synthesis of virus-specific proteins in MDCK cells. Under the same conditions, the non-modified antisense oligo and modified nonsense oligo did not affect the virus development.

Original languageEnglish (US)
Pages (from-to)327-330
Number of pages4
JournalFEBS Letters
Volume259
Issue number2
DOIs
StatePublished - Jan 1 1990

Fingerprint

Madin Darby Canine Kidney Cells
Antisense Oligonucleotides
Orthomyxoviridae
Viruses
Antiviral Agents
Reproduction
H1N1 Subtype Influenza A Virus
Influenza A virus
DNA-Directed RNA Polymerases
Oligonucleotides
Human Influenza
Proteins
Phosphates
DNA
RNA

Keywords

  • Antisense
  • Influenza
  • Inhibition
  • Oligonucleotide
  • Translation
  • Virus

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

A new class of antivirals : antisense oligonucleotides combined with a hydrophobic substituent effectively inhibit influenza virus reproduction and synthesis of virus-specific proteins in MDCK cells. / Kabanov, Alexander V.; Vinogradov, Sergei V.; Ovcharenko, Alexander V.; Krivonos, Alexander V.; Melik-Nubarov, Nikolai S.; Kiselev, Vsevolod I.; Severin, Eugenii S.

In: FEBS Letters, Vol. 259, No. 2, 01.01.1990, p. 327-330.

Research output: Contribution to journalArticle

Kabanov, Alexander V. ; Vinogradov, Sergei V. ; Ovcharenko, Alexander V. ; Krivonos, Alexander V. ; Melik-Nubarov, Nikolai S. ; Kiselev, Vsevolod I. ; Severin, Eugenii S. / A new class of antivirals : antisense oligonucleotides combined with a hydrophobic substituent effectively inhibit influenza virus reproduction and synthesis of virus-specific proteins in MDCK cells. In: FEBS Letters. 1990 ; Vol. 259, No. 2. pp. 327-330.
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