A new class of antivirals: antisense oligonucleotides combined with a hydrophobic substituent effectively inhibit influenza virus reproduction and synthesis of virus-specific proteins in MDCK cells

Alexander V. Kabanov, Sergei V. Vinogradov, Alexander V. Ovcharenko, Alexander V. Krivonos, Nikolai S. Melik-Nubarov, Vsevolod I. Kiselev, Eugenii S. Severin

Research output: Contribution to journalArticle

85 Scopus citations

Abstract

To enhance the penetration of oligonucleotide ('oligo') into cells, the oligo was combined with the hydrophobic undecyl residue. Using the 'DNA-synthesator', we synthesized oligo, complementary to the loop-forming site of the RNA, encoding polymerase 3 of the influenza virus (type A), and combined it with the undecyl residue added to the 5' terminal phosphate group. It was found that the modified oligo effectively suppresses the influenza A/PR8/34 (H1N1) virus reproduction and inhibits the synthesis of virus-specific proteins in MDCK cells. Under the same conditions, the non-modified antisense oligo and modified nonsense oligo did not affect the virus development.

Original languageEnglish (US)
Pages (from-to)327-330
Number of pages4
JournalFEBS Letters
Volume259
Issue number2
DOIs
Publication statusPublished - Jan 1 1990

    Fingerprint

Keywords

  • Antisense
  • Influenza
  • Inhibition
  • Oligonucleotide
  • Translation
  • Virus

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this