A new cholesterol derivative suitable for transfecting certain type of cells in the presence of 10% serum

Aleksander Sochanik, Irina Kaida, Iwona Mitrus, Andrzej Rajca, Stanisław Szala

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We developed a new cationic lipid suitable for use as a DNA carrier in the presence of 10% sera. The novel compound (abbreviated as Arg-Chol) contains cholesterol and a dipeptide consisting of glycine and sterically protected arginine. The efficiency of reporter gene transfection using liposomes based on this new reagent was compared with that of liposomes made with other cationic derivatives of cholesterol. Lipoplexes formulated with the newly synthesized lipid mediate in vitro transfection of B16(F10) murine melanoma cells in the presence of 10% sera more efficiently than in other cell lines and compared with other cholesterol derivatives studied.

Original languageEnglish (US)
Pages (from-to)513-520
Number of pages8
JournalCancer Gene Therapy
Volume7
Issue number4
DOIs
StatePublished - Jan 1 2000

Fingerprint

Cholesterol
Liposomes
Transfection
Serum
Lipids
Dipeptides
Reporter Genes
Glycine
Arginine
Melanoma
Cell Line
DNA
In Vitro Techniques

Keywords

  • 10% serum
  • Cationic cholesterol
  • Gene transfection

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Cancer Research

Cite this

A new cholesterol derivative suitable for transfecting certain type of cells in the presence of 10% serum. / Sochanik, Aleksander; Kaida, Irina; Mitrus, Iwona; Rajca, Andrzej; Szala, Stanisław.

In: Cancer Gene Therapy, Vol. 7, No. 4, 01.01.2000, p. 513-520.

Research output: Contribution to journalArticle

Sochanik, Aleksander ; Kaida, Irina ; Mitrus, Iwona ; Rajca, Andrzej ; Szala, Stanisław. / A new cholesterol derivative suitable for transfecting certain type of cells in the presence of 10% serum. In: Cancer Gene Therapy. 2000 ; Vol. 7, No. 4. pp. 513-520.
@article{adbef7e5d10f456a8c77c99a2889db3a,
title = "A new cholesterol derivative suitable for transfecting certain type of cells in the presence of 10{\%} serum",
abstract = "We developed a new cationic lipid suitable for use as a DNA carrier in the presence of 10{\%} sera. The novel compound (abbreviated as Arg-Chol) contains cholesterol and a dipeptide consisting of glycine and sterically protected arginine. The efficiency of reporter gene transfection using liposomes based on this new reagent was compared with that of liposomes made with other cationic derivatives of cholesterol. Lipoplexes formulated with the newly synthesized lipid mediate in vitro transfection of B16(F10) murine melanoma cells in the presence of 10{\%} sera more efficiently than in other cell lines and compared with other cholesterol derivatives studied.",
keywords = "10{\%} serum, Cationic cholesterol, Gene transfection",
author = "Aleksander Sochanik and Irina Kaida and Iwona Mitrus and Andrzej Rajca and Stanisław Szala",
year = "2000",
month = "1",
day = "1",
doi = "10.1038/sj.cgt.7700167",
language = "English (US)",
volume = "7",
pages = "513--520",
journal = "Cancer Gene Therapy",
issn = "0929-1903",
publisher = "Nature Publishing Group",
number = "4",

}

TY - JOUR

T1 - A new cholesterol derivative suitable for transfecting certain type of cells in the presence of 10% serum

AU - Sochanik, Aleksander

AU - Kaida, Irina

AU - Mitrus, Iwona

AU - Rajca, Andrzej

AU - Szala, Stanisław

PY - 2000/1/1

Y1 - 2000/1/1

N2 - We developed a new cationic lipid suitable for use as a DNA carrier in the presence of 10% sera. The novel compound (abbreviated as Arg-Chol) contains cholesterol and a dipeptide consisting of glycine and sterically protected arginine. The efficiency of reporter gene transfection using liposomes based on this new reagent was compared with that of liposomes made with other cationic derivatives of cholesterol. Lipoplexes formulated with the newly synthesized lipid mediate in vitro transfection of B16(F10) murine melanoma cells in the presence of 10% sera more efficiently than in other cell lines and compared with other cholesterol derivatives studied.

AB - We developed a new cationic lipid suitable for use as a DNA carrier in the presence of 10% sera. The novel compound (abbreviated as Arg-Chol) contains cholesterol and a dipeptide consisting of glycine and sterically protected arginine. The efficiency of reporter gene transfection using liposomes based on this new reagent was compared with that of liposomes made with other cationic derivatives of cholesterol. Lipoplexes formulated with the newly synthesized lipid mediate in vitro transfection of B16(F10) murine melanoma cells in the presence of 10% sera more efficiently than in other cell lines and compared with other cholesterol derivatives studied.

KW - 10% serum

KW - Cationic cholesterol

KW - Gene transfection

UR - http://www.scopus.com/inward/record.url?scp=0034075246&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034075246&partnerID=8YFLogxK

U2 - 10.1038/sj.cgt.7700167

DO - 10.1038/sj.cgt.7700167

M3 - Article

VL - 7

SP - 513

EP - 520

JO - Cancer Gene Therapy

JF - Cancer Gene Therapy

SN - 0929-1903

IS - 4

ER -