A new approach for identification of novel antihypertensive peptides from egg proteins by QSAR and bioinformatics

Kaustav Majumder, Jianping Wu

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Many protein-derived bioactive peptides were identified after extensive activity-guided purification, which is labor-intensive and costly. Furthermore, the rationale behind the selection of a substrate protein and a protease over others has not been justified in literature. The purpose of the study was to explore the rationale behind the selection of conditions for the production of potent angiotensin I converting enzyme (ACE) inhibitory peptides from egg proteins. Based on in silico digestion and quantitative structure and activity relationship (QSAR) model prediction, thermolysin-pepsin digestion of ovotransferrin was chosen as the best condition due to the presence of three potent peptides, Ile-Arg-Try, Leu-Lys-Pro and Ile-Gln-Try. To our surprise, sequences of Ile-Arg-Try-Cys-Thr, Leu-Lys-Pro-Ile and Ile-Gln-Try-Cys-Ala, but not Ile-Arg-Try, Leu-Lys-Pro and Ile-Gln-Try, were present in the hydrolysate. Further study showed that sonication or reducing agent pre-treatments could improve the activity of hydrolysates over 20 times and the predicted peptides were successfully released from sonication-treated ovotransferrin hydrolysate.

Original languageEnglish (US)
Pages (from-to)1371-1378
Number of pages8
JournalFood Research International
Volume43
Issue number5
DOIs
StatePublished - Jun 1 2010

Fingerprint

Egg Proteins
egg proteins
antihypertensive agents
Quantitative Structure-Activity Relationship
Computational Biology
bioinformatics
Antihypertensive Agents
Conalbumin
hydrolysates
peptides
Peptides
Sonication
Digestion
digestion
Thermolysin
reducing agents
Pepsin A
Reducing Agents
peptidyl-dipeptidase A
Peptidyl-Dipeptidase A

Keywords

  • ACE inhibitory peptides
  • Bioinformatics
  • Egg proteins
  • Ovotransferrin
  • QSAR
  • Sonication

ASJC Scopus subject areas

  • Food Science

Cite this

A new approach for identification of novel antihypertensive peptides from egg proteins by QSAR and bioinformatics. / Majumder, Kaustav; Wu, Jianping.

In: Food Research International, Vol. 43, No. 5, 01.06.2010, p. 1371-1378.

Research output: Contribution to journalArticle

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