A nested set of six or seven subgenomic mRNAs is formed in cells infected with different isolates of porcine reproductive and respiratory syndrome virus

Xiang Jin Meng, Prem S Paul, Igor Morozov, Patrick G. Halbur

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Abstract

The subgenomic mRNAs (sg mRNA) of porcine reproductive and respiratory syndrome virus (PRRSV) were characterized. The number of sg mRNAs, which form a 3'-coterminal nested set in PRRSV-infected cells, varied from six to seven among PRRSV isolates with differing virulence. The additional species of sg mRNA in some isolates of PRRSV was designated as sg mRNA 3-1. The leader-mRNA junction sequences of sg mRNAs 3, 3-1 and 4 were found to contain a similar six nucleotide sequence motif, U(G)UA(G/C)ACC. By comparing the 5'-terminal sequence of sg mRNA 3-1 with the genomic sequence of two isolates, ISU79 and ISU1894, it was found that a point mutation, from U in isolate ISU1894 to C in isolate ISU79, led to the acquisition of a new leader-mRNA junction sequence (UUGACC) in isolate ISU79, and therefore an additional species of sg mRNA 3-1. A small ORF (3-1) was identified at the 5' end of sg mRNA 3-1.

Original languageEnglish (US)
Pages (from-to)1265-1270
Number of pages6
JournalJournal of General Virology
Volume77
Issue number6
DOIs
StatePublished - Jan 1 1996

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Porcine respiratory and reproductive syndrome virus
Messenger RNA
5' Untranslated Regions
Nucleotide Motifs
Point Mutation
Open Reading Frames
Virulence

ASJC Scopus subject areas

  • Virology

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A nested set of six or seven subgenomic mRNAs is formed in cells infected with different isolates of porcine reproductive and respiratory syndrome virus. / Meng, Xiang Jin; Paul, Prem S; Morozov, Igor; Halbur, Patrick G.

In: Journal of General Virology, Vol. 77, No. 6, 01.01.1996, p. 1265-1270.

Research output: Contribution to journalArticle

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