A nanofibrous composite membrane of PLGA-chitosan/PVA prepared by electrospinning

Bin Duan, Xiaoyan Yuan, Yi Zhu, Yuanyuan Zhang, Xiulan Li, Yang Zhang, Kangde Yao

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184 Scopus citations

Abstract

Tissue engineering scaffolds produced by electrospinning feature a structural similarity to the natural extracellular matrix. In this study, poly(lactide-co-glycolide) (PLGA) and chitosan/poly(vinyl alcohol) (PVA) were simultaneously electrospun from two different syringes and mixed on the rotating drum to prepare the nanofibrous composite membrane. The composite membrane was crosslinked by glutaraldehyde vapor to maintain its mechanical properties and fiber morphology in wet stage. Morphology, shrinkage, absorption in phosphate buffered solution (PBS) and mechanical properties of the electrospun membranes were characterized. Fibroblast viability on electrospun membranes was discussed by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay and cell morphology after 7 days of culture. Results indicated that the PBS absorption of the composite membranes, no matter crosslinked or not, was higher than the electrospun PLGA membrane due to the introduction of hydrophilic components, chitosan and PVA. After crosslinking, the composite membrane had a little shrinkage after incubating in PBS. The crosslinked composite membrane also showed moderate tensile properties. Cell culture suggested that electrospun PLGA-chitosan/PVA membrane tended to promote fibroblast attachment and proliferation. It was assumed that the nanofibrous composite membrane of electrospun PLGA-chitosan/PVA could be potentially used for skin reconstruction.

Original languageEnglish (US)
Pages (from-to)2013-2022
Number of pages10
JournalEuropean Polymer Journal
Volume42
Issue number9
DOIs
StatePublished - Sep 1 2006

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Keywords

  • Chitosan
  • Electrospinning
  • Fibroblast
  • Nanofibrous composite membrane
  • Poly(lactide-co-glycolide)

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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