A mutation linked with autism reveals a common mechanism of endoplasmic reticulum retention for the α,β-hydrolase fold protein family

Antonella De Jaco, Davide Comoletti, Zrinka Kovarik, Guido Gaietta, Zoran Radić, Oksana Lockridge, Mark H. Ellisman, Palmer Taylor

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

A mutation linked to autistic spectrum disorders encodes an Arg to Cys replacement in the C-terminal portion of the extracellular domain of neuroligin-3. The solvent-exposed Cys causes virtually complete retention of the protein in the endoplasmic reticulum when the protein is expressed in transfected cells. An identical Cys substitution was reported for butyrylcholinesterase through genotyping patients with post-succinylcholine apnea. Neuroligin, butyrylcholinesterase, and acetylcholinesterase are members of the α,β-hydrolase fold family of proteins sharing sequence similarity and common tertiary structures. Although these proteins have distinct oligomeric assemblies and cellular dispositions, homologous Arg residues in neuroligin-3 (Arg-451), in butyrylcholinesterase (Arg-386), and in acetylcholinesterase (Arg-395) are conserved in all studied mammalian species. To examine whether an homologous Arg to Cys mutation affects related proteins similarly despite their differing capacities to oligomerize, we inserted homologous mutations in the acetylcholinesterase and butyrylcholinesterase cDNAs. Using confocal fluorescence microscopy and analysis of oligosaccharide processing, we find that the homologous Arg to Cys mutation also results in endoplasmic reticulum retention of the two cholinesterases. Small quantities of mutated acetylcholinesterase exported from the cell retain activity but show a greater Km, a much smaller kcat, and altered substrate inhibition. The nascent proteins associate with chaperones during processing, but the mutation presumably restricts processing through the endoplasmic reticulum and Golgi apparatus, because of local protein misfolding and inability to oligomerize. The mutation may alter the capacity of these proteins to dissociate from their chaperone prior to oligomerization and processing for export.

Original languageEnglish (US)
Pages (from-to)9667-9676
Number of pages10
JournalJournal of Biological Chemistry
Volume281
Issue number14
DOIs
StatePublished - Apr 7 2006

Fingerprint

Hydrolases
Autistic Disorder
Endoplasmic Reticulum
Butyrylcholinesterase
Mutation
Acetylcholinesterase
Proteins
Processing
Oligomerization
Succinylcholine
Retention (Psychology)
Confocal microscopy
Fluorescence microscopy
Cholinesterases
Golgi Apparatus
Apnea
Oligosaccharides
Fluorescence Microscopy
Confocal Microscopy
Substitution reactions

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

A mutation linked with autism reveals a common mechanism of endoplasmic reticulum retention for the α,β-hydrolase fold protein family. / De Jaco, Antonella; Comoletti, Davide; Kovarik, Zrinka; Gaietta, Guido; Radić, Zoran; Lockridge, Oksana; Ellisman, Mark H.; Taylor, Palmer.

In: Journal of Biological Chemistry, Vol. 281, No. 14, 07.04.2006, p. 9667-9676.

Research output: Contribution to journalArticle

De Jaco, Antonella ; Comoletti, Davide ; Kovarik, Zrinka ; Gaietta, Guido ; Radić, Zoran ; Lockridge, Oksana ; Ellisman, Mark H. ; Taylor, Palmer. / A mutation linked with autism reveals a common mechanism of endoplasmic reticulum retention for the α,β-hydrolase fold protein family. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 14. pp. 9667-9676.
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