A Mutation in the Latency-Related Gene of Bovine Herpesvirus 1 Inhibits Protein Expression from Open Reading Frame 2 and an Adjacent Reading Frame during Productive Infection

Yunquan Jiang, Melissa Inman, Yange Zhang, Nuria Alemañ Posadas, Clinton Jones

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

The latency-related (LR) gene of bovine herpesvirus 1 (BHV-1) is abundantly expressed during latency. A mutant BHV-1 strain that contains three stop codons at the 5′ terminus of the LR gene (LR mutant) does not reactivate from latency. This study demonstrates that the LR mutant does not express open reading frame 2 or an adjacent reading frame that lacks an initiating ATG (reading frame C). Since the LR mutant and wild-type BHV-1 express similar levels of LR RNA, we conclude that LR protein expression plays an important role in regulating the latency reactivation cycle in cattle.

Original languageEnglish (US)
Pages (from-to)3184-3189
Number of pages6
JournalJournal of virology
Volume78
Issue number6
DOIs
Publication statusPublished - Mar 1 2004

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ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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