A mutation in the hamster α(1B)-adrenergic receptor that differentiates two steps in the pathway of receptor internalization

Jiefa Wang, Jialin C Zheng, Jodi L. Anderson, Myron Lee Toews

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

An NP(X)(n)Y motif is highly conserved among G protein-coupled receptors and is similar to an NPXY motif involved in receptor-mediated endocytosis for several non-G protein-coupled receptors. We investigated the role of this motif in α(1B)-adrenergic receptor function and regulation. Y348A α(1B)- adrenergic receptors in which this sequence was mutated from NPIIY to NPIIA were prepared by site-directed mutagenesis and transfected into Chinese hamster ovary cells. Binding of the antagonist prazosin to Y348A receptors was similar to that of wild-type receptors, but affinity of the Y348A receptors for the agonist epinephrine was increased by ~10-fold. Despite this increase in agonist binding affinity, the Y348A mutation completely uncoupled the receptors from stimulation of phosphoinositide hydrolysis and mobilization of intracellular Ca2+. Exposure of cells expressing Y348A receptors to the agonist epinephrine resulted in receptor 'sequestration,' defined as a toss of cell surface receptors accessible to radio-ligand in binding assays with intact cells on ice, similar to that for the wild-type receptor. In contrast, Y348A receptors did not undergo 'endocytosis' into the light vesicle fraction in sucrose density gradient centrifugation assays, as did the wild-type receptor. These results (i) indicate an important role for Tyr348 in coupling the α(1B)-adrenergic receptor to G protein and subsequent effector activation, (ii) provide further evidence that α(1B)-adrenergic receptor internalization can be separated into a sequestration step and an endocytosis step, (iii) indicate that effector activation and second messenger formation are not required for the sequestration of these receptors but may be involved in endocytosis, and (iv) provide a useful new tool for further investigation of the nature of the subcellular compartments and the molecular modifications involved in the multiple steps involved in internalization of G protein-coupled receptors.

Original languageEnglish (US)
Pages (from-to)306-313
Number of pages8
JournalMolecular pharmacology
Volume52
Issue number2
DOIs
StatePublished - Aug 1997

Fingerprint

Cricetinae
Adrenergic Receptors
Endocytosis
Mutation
G-Protein-Coupled Receptors
Density Gradient Centrifugation
Prazosin
Ice
Cell Surface Receptors
Second Messenger Systems
Site-Directed Mutagenesis
Cricetulus
Radio
GTP-Binding Proteins
Epinephrine
Sucrose
Ovary
Hydrolysis
Ligands
Light

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

A mutation in the hamster α(1B)-adrenergic receptor that differentiates two steps in the pathway of receptor internalization. / Wang, Jiefa; Zheng, Jialin C; Anderson, Jodi L.; Toews, Myron Lee.

In: Molecular pharmacology, Vol. 52, No. 2, 08.1997, p. 306-313.

Research output: Contribution to journalArticle

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