A multienzyme complex channels substrates and electrons through acetyl-coA and methane biosynthesis pathways in Methanosarcina

Dillon J. Lieber, Jennifer Catlett, Nandu Madayiputhiya, Renu Nandakumar, Madeline M. Lopez, William W. Metcalf, Nicole R. Buan

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Multienzyme complexes catalyze important metabolic reactions in many organisms, but little is known about the complexes involved in biological methane production (methanogenesis). A crosslinking-mass spectrometry (XL-MS) strategy was employed to identify proteins associated with coenzyme M-coenzyme B heterodisulfide reductase (Hdr), an essential enzyme in all methane-producing archaea (methanogens). In Methanosarcina acetivorans, Hdr forms a multienzyme complex with acetyl-CoA decarbonylase synthase (ACDS), and F420-dependent methylene-H4MPT reductase (Mer). ACDS is essential for production of acetyl-CoA during growth on methanol, or for methanogenesis from acetate, whereas Mer is essential for methanogenesis from all substrates. Existence of a Hdr:ACDS:Mer complex is consistent with growth phenotypes of ACDS and Mer mutant strains in which the complex samples the redox status of electron carriers and directs carbon flux to acetyl-CoA or methanogenesis. We propose the Hdr:ACDS:Mer complex comprises a special class of multienzyme redox complex which functions as a "biological router" that physically links methanogenesis and acetyl-CoA biosynthesis pathways.

Original languageEnglish (US)
Article numbere107563
JournalPloS one
Volume9
Issue number9
DOIs
StatePublished - Sep 18 2014

Fingerprint

Methanosarcina
multienzyme complexes
Multienzyme Complexes
Acetyl Coenzyme A
Biosynthesis
Methane
methane production
electrons
Electrons
Substrates
Oxidoreductases
coenzymes
Oxidation-Reduction
methanogens
Mesna
Methanogens
Archaea
crosslinking
Carbon Cycle
methane

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

A multienzyme complex channels substrates and electrons through acetyl-coA and methane biosynthesis pathways in Methanosarcina. / Lieber, Dillon J.; Catlett, Jennifer; Madayiputhiya, Nandu; Nandakumar, Renu; Lopez, Madeline M.; Metcalf, William W.; Buan, Nicole R.

In: PloS one, Vol. 9, No. 9, e107563, 18.09.2014.

Research output: Contribution to journalArticle

Lieber, Dillon J. ; Catlett, Jennifer ; Madayiputhiya, Nandu ; Nandakumar, Renu ; Lopez, Madeline M. ; Metcalf, William W. ; Buan, Nicole R. / A multienzyme complex channels substrates and electrons through acetyl-coA and methane biosynthesis pathways in Methanosarcina. In: PloS one. 2014 ; Vol. 9, No. 9.
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