A monoclonal antibody sandwich immunoassay for serum amyloid A (SAA) protein

Thomas L. McDonald, Annika Weber, James W. Smith

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

An antibody sandwich immunoassay using two purified rat monoclonal antibodies to human serum amyloid A was developed and used to measure serum amyloid A in human serum. The assay was specific, sensitive, reproducible, and reliable and does not require denaturation of the specimen prior to assay. Serum amyloid A purified by hydrophobic interaction chromatography of acute phase human serum afforded a reliable standard for the assay. A significant (r = 0.69) correlation for SAA and C reactive protein values was found for 180 patient samples analyzed.

Original languageEnglish (US)
Pages (from-to)149-155
Number of pages7
JournalJournal of Immunological Methods
Volume144
Issue number2
DOIs
StatePublished - Nov 22 1991

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Serum Amyloid A Protein
Immunoassay
Monoclonal Antibodies
Serum
Hydrophobic and Hydrophilic Interactions
C-Reactive Protein
Chromatography
Antibodies

Keywords

  • C reactive protein
  • ELISA
  • Monoclonal antibody
  • Serum amyloid A

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

A monoclonal antibody sandwich immunoassay for serum amyloid A (SAA) protein. / McDonald, Thomas L.; Weber, Annika; Smith, James W.

In: Journal of Immunological Methods, Vol. 144, No. 2, 22.11.1991, p. 149-155.

Research output: Contribution to journalArticle

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