A molecular phylogeny for the Drosophila melanogaster subgroup and the problem of polymorphism data

Adalgisa Caccone, Etsuko N. Moriyama, Jennifer M. Gleason, Loredana Nigro, Jeffrey R. Powell

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Drosophila melanogaster belongs to a closely related group of eight species collectively known as the melanogaster subgroup; all are native to sub-Saharan Africa and islands off the east coast of Africa. The phylogenetic relationships of most species in this subgroup have been well documented; however, the three most closely related species. D. simulans, D. sechellia, and D. mauritiana, have remained problematic from a phylogenetic standpoint as no data set has unambiguously resolved them. We present new DNA sequence data on the nullo and Serendipity-α genes and combine them with all available nuclear DNA sequence data; the total data encompass 12 genes and the ITS of rDNA. A methodological problem arose because nine of the genes had information on intraspecific polymorphisms in at least one species. We explored the effect of inclusion/exclusion of polymorphic sites and found that it had very little effect on phylogenetic inferences, due largely to the fact that 82% of polymorphisms are autapomorphies (unique to one species). We have also reanalyzed our previous DNA-DNA hybridization data with a bootstrap procedure. The combined sequence data set and the DNA-DNA hybridization data strongly support the sister status of the two island species, D. sechellia and D. mauritiana. This at least partially resolves what had been a paradox of parallel evolution in these two species.

Original languageEnglish (US)
Pages (from-to)1224-1232
Number of pages9
JournalMolecular biology and evolution
Volume13
Issue number9
DOIs
StatePublished - Nov 1996

Fingerprint

Phylogeny
Drosophila melanogaster
Polymorphism
phylogeny
polymorphism
genetic polymorphism
nucleic acid hybridization
Genes
DNA sequences
DNA
Islands
nucleotide sequences
parallel evolution
Melanogaster
genes
Sub-Saharan Africa
Eastern Africa
nuclear genome
Africa South of the Sahara
phylogenetics

Keywords

  • Drosophila melanogaster subgroup
  • Sry-α
  • molecular phylogenies
  • nullo

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Cite this

A molecular phylogeny for the Drosophila melanogaster subgroup and the problem of polymorphism data. / Caccone, Adalgisa; Moriyama, Etsuko N.; Gleason, Jennifer M.; Nigro, Loredana; Powell, Jeffrey R.

In: Molecular biology and evolution, Vol. 13, No. 9, 11.1996, p. 1224-1232.

Research output: Contribution to journalArticle

Caccone, Adalgisa ; Moriyama, Etsuko N. ; Gleason, Jennifer M. ; Nigro, Loredana ; Powell, Jeffrey R. / A molecular phylogeny for the Drosophila melanogaster subgroup and the problem of polymorphism data. In: Molecular biology and evolution. 1996 ; Vol. 13, No. 9. pp. 1224-1232.
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