A model for the role of EHD1-containing membrane tubules in endocytic recycling

Mahak Sharma, Marko Jovic, Fabien Kieken, Naava Naslavsky, Paul Sorgen, Steve Caplan

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The C-terminal Eps15 homology domain-containing protein, EHD1, is an important regulator of receptor recycling back to the plasma membrane. In addition to its vesicular localization, EHD1 also localizes to a unique array of tubular membrane structures that emanate from the endocytic recycling compartment. While these structures have been described over seven years ago, addressing their lipid composition and physiological function has been challenging. Moreover, it was not known whether EHD1 itself induces tubule formation, or whether it localizes to pre-existing tubular membrane structures. We have demonstrated that in vivo, EHD1 localizes to pre-existing tubular membranes that contain both phosphatidylinositol-4-phosphate and phosphatidylinositol-(4,5)-bisphosphate. Moreover, we have determined that 'non-tubular' EHD1 mutants with a single residue substitution do not efficiently facilitate receptor recycling. Our data suggest that EHD1-associated tubules are required for efficient recycling and we propose models that describe the potential mechanisms by which EHD1 functions.

Original languageEnglish (US)
Pages (from-to)431-433
Number of pages3
JournalCommunicative and Integrative Biology
Volume2
Issue number5
DOIs
StatePublished - Dec 1 2009

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Keywords

  • EHD1
  • Endocytic recycling compartment
  • Phosphatidylinositol-(4,5)-bisphosphate
  • Phosphatidylinositol-4-phosphate
  • Tubules

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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