A mitochondrial DNA sequence is associated with abnormal pollen development in cytoplasmic male sterile bean plants

Carol Johns, Meiqing Lu, Anna Lyznik, Sally Mackenzie

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Cytoplasmic male sterility (CMS) in common bean is associated with the presence of a 3-kb unique mitochondrial sequence designated pvs. The pvs sequence encodes at least two open reading frames (297 and 720 bp in length) with portions derived from the chloroplast genome. Fertility restoration by the nuclear restorer gene Fr results in the loss of this transcriptionally active unique region. We examined the effect of CMS (pvs present) and fertility restoration by Fr (pvs absent) on the pattern of pollen development in bean. In the CMS line, pollen aborted in the tetrad stage late in microgametogenesis. Microspores maintained cytoplasmic connections throughout pollen development, indicating aberrant or incomplete cytokinesis. Pollen-specific events associated with pollen abortion and fertility restoration imply that a gametophytic factor or event may be involved in CMS. In situ hybridization experiments suggested that significant reduction or complete loss of the mitochondrial sterility-associated sequence occurred in fertile pollen of F2 populations segregating for fertility. These observations support a model of fertility restoration by the loss of a mitochondrial DNA sequence prior to or during microsporogenesis/gametogenesis.

Original languageEnglish (US)
Pages (from-to)435-449
Number of pages15
JournalPlant Cell
Volume4
Issue number4
StatePublished - Apr 1 1992

Fingerprint

DNA sequences
Pollen
Mitochondrial DNA
cytoplasmic male sterility
Restoration
beans
mitochondrial DNA
Male Infertility
Fertility
pollen
nucleotide sequences
Genes
Plant Gametogenesis
microsporogenesis
abortion (plants)
Chloroplast Genome
Gametogenesis
cytokinesis
gametogenesis
microspores

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

A mitochondrial DNA sequence is associated with abnormal pollen development in cytoplasmic male sterile bean plants. / Johns, Carol; Lu, Meiqing; Lyznik, Anna; Mackenzie, Sally.

In: Plant Cell, Vol. 4, No. 4, 01.04.1992, p. 435-449.

Research output: Contribution to journalArticle

Johns, Carol ; Lu, Meiqing ; Lyznik, Anna ; Mackenzie, Sally. / A mitochondrial DNA sequence is associated with abnormal pollen development in cytoplasmic male sterile bean plants. In: Plant Cell. 1992 ; Vol. 4, No. 4. pp. 435-449.
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