A method for the making and utility of gadolinium-labeled albumin microbubbles

Daniel R Anderson, Michael J. Duryee, Robert P. Garvin, Michael D. Boska, Geoffrey Milton Thiele, Lynell Warren Klassen

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objective: Perfluorocarbon-exposed sonicated dextrose albumin microbubbles (PESDA) binds scavenger receptors and can be noninvasively imaged. To enhance imaging, gadolinium (Gd)-labeled PESDA was developed and tested in a model of vascular inflammation by magnetic resonance imaging (MRI). Methods and Results: Purified human serum albumin (HSA) (5%) was labeled with Gd via the covalent binding of diethylenetriaminepentacetic acid. Abdominal aortic tissues in Sprague-Dawley rats (n=5 per group) were analyzed by 7-T MRI and scanning electron microscopy to evaluate PESDA binding. Labeling-purified 5% human albumin resulted in an average of 16.1 Gd atoms per albumin molecule as determined by atomic absorption. Forty-eight hours after balloon angioplasty, aortic tissue was enhanced with Gd-PESDA as compared to control tissue. 7-T MRI of explanted tissues was sensitive to the detection of retained PESDA. Enhancement of aortic tissue in vivo was present albeit to a lesser extent than explanted tissue from the same animals. Conclusions: HSA was successfully labeled, and an albumin-based microbubble with Gd was synthesized. This contrast agent, Gd-PESDA, may serve as an additional agent for the MRI evaluation of innate inflammation and used to noninvasively image early vascular pathophysiologic processes. Condensed Abstract: In this study, Gd-PESDA microbubbles and were synthesized and shown to detect the binding of these microbubbles using MRI in injured aortic tissue. The method for synthesizing Gd-PESDA is detailed, and the proposed utility of this new contrast agent is discussed.

Original languageEnglish (US)
Pages (from-to)96-103
Number of pages8
JournalMagnetic Resonance Imaging
Volume30
Issue number1
DOIs
StatePublished - Jan 1 2012

Fingerprint

Dextrose
Microbubbles
Gadolinium
Fluorocarbons
Albumins
Magnetic resonance
Tissue
Imaging techniques
Glucose
Magnetic Resonance Imaging
Serum Albumin
Contrast Media
Blood Vessels
Balloons
Inflammation
Scavenger Receptors
Labeling
Rats
Balloon Angioplasty
Animals

Keywords

  • Atherosclerosis
  • Gadolinium
  • MRI
  • Magnetic Resonance Imaging
  • PESDA
  • Perfluorocarbon exposed sonicated dextrose albumin
  • Scavenger receptors
  • Vascular inflammation

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

Cite this

A method for the making and utility of gadolinium-labeled albumin microbubbles. / Anderson, Daniel R; Duryee, Michael J.; Garvin, Robert P.; Boska, Michael D.; Thiele, Geoffrey Milton; Klassen, Lynell Warren.

In: Magnetic Resonance Imaging, Vol. 30, No. 1, 01.01.2012, p. 96-103.

Research output: Contribution to journalArticle

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abstract = "Objective: Perfluorocarbon-exposed sonicated dextrose albumin microbubbles (PESDA) binds scavenger receptors and can be noninvasively imaged. To enhance imaging, gadolinium (Gd)-labeled PESDA was developed and tested in a model of vascular inflammation by magnetic resonance imaging (MRI). Methods and Results: Purified human serum albumin (HSA) (5{\%}) was labeled with Gd via the covalent binding of diethylenetriaminepentacetic acid. Abdominal aortic tissues in Sprague-Dawley rats (n=5 per group) were analyzed by 7-T MRI and scanning electron microscopy to evaluate PESDA binding. Labeling-purified 5{\%} human albumin resulted in an average of 16.1 Gd atoms per albumin molecule as determined by atomic absorption. Forty-eight hours after balloon angioplasty, aortic tissue was enhanced with Gd-PESDA as compared to control tissue. 7-T MRI of explanted tissues was sensitive to the detection of retained PESDA. Enhancement of aortic tissue in vivo was present albeit to a lesser extent than explanted tissue from the same animals. Conclusions: HSA was successfully labeled, and an albumin-based microbubble with Gd was synthesized. This contrast agent, Gd-PESDA, may serve as an additional agent for the MRI evaluation of innate inflammation and used to noninvasively image early vascular pathophysiologic processes. Condensed Abstract: In this study, Gd-PESDA microbubbles and were synthesized and shown to detect the binding of these microbubbles using MRI in injured aortic tissue. The method for synthesizing Gd-PESDA is detailed, and the proposed utility of this new contrast agent is discussed.",
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