A membrane form of TNF-α presented by exosomes delays T cell activation-induced cell death

Huang Ge Zhang, Cunren Liu, Kaihong Su, Shaohua Yu, Liming Zhang, Shuangqin Zhang, Jianhua Wang, Xu Cao, William Grizzle, Robert P. Kimberly

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

In common with many other cell types, synovial fibroblasts produce exosomes. In this study, we show that the exosomes produced by synovial fibroblasts obtained from individuals with rheumatoid arthritis (RASF), but not exosomes produced by synovial fibroblasts obtained from individuals with osteoarthritis, contain a membrane bound form of TNF-α as demonstrated by colloidal gold immunostaining of TNF-α and confirmed by both Western blot and mass spectrometry. The RASF-derived exosomes, but not exosomes derived from fibroblasts obtained from individuals with osteoarthritis, are cytotoxic for the L929 cell, a TNF-α-sensitive cell line, and stimulate activation of NF-κB and induction of collagenase-1 in RASF. These elects are blocked by addition of soluble TNFR1 (sTNFbp), suggesting that a TNF-α-signaling pathway mediates these biological activities. sTNFbp also reduced the production of exosomes by RASF, suggesting the interruption of a positive amplification loop. Exosomes can transmit signals between cells, and RASF exosomes, effectively taken up by anti-CB3-activated T cells, activated AKT and NF-κB and rendered these activated T cells resistant to apoptosis. Neutralization of exosomal membrane TNF-α by sTNFbp partially reversed this resistance, suggesting that not only TNF-α but also additional exosomal proteins may contribute to the development of apoptosis resistance.

Original languageEnglish (US)
Pages (from-to)7385-7393
Number of pages9
JournalJournal of Immunology
Volume176
Issue number12
StatePublished - Jun 15 2006

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Exosomes
Cell Death
T-Lymphocytes
Membranes
Fibroblasts
Osteoarthritis
Apoptosis
Receptors, Tumor Necrosis Factor, Type I
Gold Colloid
Mass Spectrometry
Rheumatoid Arthritis
Western Blotting
Cell Line

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Zhang, H. G., Liu, C., Su, K., Yu, S., Zhang, L., Zhang, S., ... Kimberly, R. P. (2006). A membrane form of TNF-α presented by exosomes delays T cell activation-induced cell death. Journal of Immunology, 176(12), 7385-7393.

A membrane form of TNF-α presented by exosomes delays T cell activation-induced cell death. / Zhang, Huang Ge; Liu, Cunren; Su, Kaihong; Yu, Shaohua; Zhang, Liming; Zhang, Shuangqin; Wang, Jianhua; Cao, Xu; Grizzle, William; Kimberly, Robert P.

In: Journal of Immunology, Vol. 176, No. 12, 15.06.2006, p. 7385-7393.

Research output: Contribution to journalArticle

Zhang, HG, Liu, C, Su, K, Yu, S, Zhang, L, Zhang, S, Wang, J, Cao, X, Grizzle, W & Kimberly, RP 2006, 'A membrane form of TNF-α presented by exosomes delays T cell activation-induced cell death', Journal of Immunology, vol. 176, no. 12, pp. 7385-7393.
Zhang HG, Liu C, Su K, Yu S, Zhang L, Zhang S et al. A membrane form of TNF-α presented by exosomes delays T cell activation-induced cell death. Journal of Immunology. 2006 Jun 15;176(12):7385-7393.
Zhang, Huang Ge ; Liu, Cunren ; Su, Kaihong ; Yu, Shaohua ; Zhang, Liming ; Zhang, Shuangqin ; Wang, Jianhua ; Cao, Xu ; Grizzle, William ; Kimberly, Robert P. / A membrane form of TNF-α presented by exosomes delays T cell activation-induced cell death. In: Journal of Immunology. 2006 ; Vol. 176, No. 12. pp. 7385-7393.
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