A mass spectrometric-derived cell surface protein atlas

Damaris Bausch-Fluck, Andreas Hofmann, Thomas Bock, Andreas P. Frei, Ferdinando Cerciello, Andrea Jacobs, Hansjoerg Moest, Ulrich Omasits, Rebekah L. Gundry, Charles Yoon, Ralph Schiess, Alexander Schmidt, Paulina Mirkowska, Anetta Härtlová, Jennifer E. Van Eyk, Jean Pierre Bourquin, Ruedi Aebersold, Kenneth R. Boheler, Peter Zandstra, Bernd Wollscheid

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Cell surface proteins are major targets of biomedical research due to their utility as cellular markers and their extracellular accessibility for pharmacological intervention. However, information about the cell surface protein repertoire (the surfaceome) of individual cells is only sparsely available. Here, we applied the Cell Surface Capture (CSC) technology to 41 human and 31 mouse cell types to generate a mass-spectrometry derived Cell Surface Protein Atlas (CSPA) providing cellular surfaceome snapshots at high resolution. The CSPA is presented in form of an easy-to-navigate interactive database, a downloadable data matrix and with tools for targeted surfaceome rediscovery (http://wlab.ethz.ch/cspa). The cellular surfaceome snapshots of different cell types, including cancer cells, resulted in a combined dataset of 1492 human and 1296 mouse cell surface glycoproteins, providing experimental evidence for their cell surface expression on different cell types, including 136 G-protein coupled receptors and 75 membrane receptor tyrosine-protein kinases. Integrated analysis of the CSPA reveals that the concerted biological function of individual cell types is mainly guided by quantitative rather than qualitative surfaceome differences. The CSPA will be useful for the evaluation of drug targets, for the improved classification of cell types and for a better understanding of the surfaceome and its concerted biological functions in complex signaling microenvironments.

Original languageEnglish (US)
Article numbere0121314
JournalPloS one
Volume10
Issue number4
DOIs
StatePublished - Apr 20 2015

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Atlases
surface proteins
Membrane Proteins
cells
Membrane Glycoproteins
Receptor Protein-Tyrosine Kinases
G-Protein-Coupled Receptors
Mass spectrometry
Drug Evaluation
Cells
Membranes
receptor protein-tyrosine kinase
drug evaluation
Biomedical Research
Mass Spectrometry
Pharmaceutical Preparations
Databases
Pharmacology
Technology
biomedical research

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Bausch-Fluck, D., Hofmann, A., Bock, T., Frei, A. P., Cerciello, F., Jacobs, A., ... Wollscheid, B. (2015). A mass spectrometric-derived cell surface protein atlas. PloS one, 10(4), [e0121314]. https://doi.org/10.1371/journal.pone.0121314

A mass spectrometric-derived cell surface protein atlas. / Bausch-Fluck, Damaris; Hofmann, Andreas; Bock, Thomas; Frei, Andreas P.; Cerciello, Ferdinando; Jacobs, Andrea; Moest, Hansjoerg; Omasits, Ulrich; Gundry, Rebekah L.; Yoon, Charles; Schiess, Ralph; Schmidt, Alexander; Mirkowska, Paulina; Härtlová, Anetta; Van Eyk, Jennifer E.; Bourquin, Jean Pierre; Aebersold, Ruedi; Boheler, Kenneth R.; Zandstra, Peter; Wollscheid, Bernd.

In: PloS one, Vol. 10, No. 4, e0121314, 20.04.2015.

Research output: Contribution to journalArticle

Bausch-Fluck, D, Hofmann, A, Bock, T, Frei, AP, Cerciello, F, Jacobs, A, Moest, H, Omasits, U, Gundry, RL, Yoon, C, Schiess, R, Schmidt, A, Mirkowska, P, Härtlová, A, Van Eyk, JE, Bourquin, JP, Aebersold, R, Boheler, KR, Zandstra, P & Wollscheid, B 2015, 'A mass spectrometric-derived cell surface protein atlas', PloS one, vol. 10, no. 4, e0121314. https://doi.org/10.1371/journal.pone.0121314
Bausch-Fluck D, Hofmann A, Bock T, Frei AP, Cerciello F, Jacobs A et al. A mass spectrometric-derived cell surface protein atlas. PloS one. 2015 Apr 20;10(4). e0121314. https://doi.org/10.1371/journal.pone.0121314
Bausch-Fluck, Damaris ; Hofmann, Andreas ; Bock, Thomas ; Frei, Andreas P. ; Cerciello, Ferdinando ; Jacobs, Andrea ; Moest, Hansjoerg ; Omasits, Ulrich ; Gundry, Rebekah L. ; Yoon, Charles ; Schiess, Ralph ; Schmidt, Alexander ; Mirkowska, Paulina ; Härtlová, Anetta ; Van Eyk, Jennifer E. ; Bourquin, Jean Pierre ; Aebersold, Ruedi ; Boheler, Kenneth R. ; Zandstra, Peter ; Wollscheid, Bernd. / A mass spectrometric-derived cell surface protein atlas. In: PloS one. 2015 ; Vol. 10, No. 4.
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