A LysR-type regulator, CidR, is required for induction of the Staphylococcus aureus cidABC operon

Soo Jin Yang, Kelly C. Rice, Raquel J. Brown, Toni G. Patton, Linda E. Liou, Yong Ho Park, Kenneth W Bayles

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The Staphylococcus aureus cidABC and lrgAB operons have been shown to regulate murein hydrolase activity and affect antibiotic tolerance. The cid operon enhances murein hydrolase activity and antibiotic sensitivity, whereas the lrg operon inhibits these processes. Based on these findings and the structural similarities of the cidA and lrgA gene products to the bacteriophage holin family of proteins, we have proposed that the cid and lrg operons encode holin- and antiholin-like proteins, respectively, that function to control the murein hydrolase activity produced by the bacteria. Analysis of cid operon transcription revealed the presence of two transcripts, one spanning all three cid genes and whose expression is induced by growth in the presence of acetic acid and the other spanning cid B and cidC only that is produced in a sigma B-dependent manner. The cidABC operon lies immediately downstream from the cidR gene, encoding a potential LysR-type transcriptional regulator. In this study, we demonstrate that cidR is involved in the regulation of cidABC expression. Northern blot analyses revealed that the cidR gene product positively regulates cidABC expression by increasing transcription in the presence of acetic acid produced as a result of the metabolism of glucose. As expected for an operon that encodes a positive effector of murein hydrolase activity, the upregulation of cidABC expression resulted in increased murein hydrolase activity produced by these cells. Furthermore, it was demonstrated that antibiotic tolerance and stationary-phase survival of S. aureus are affected by the cidR gene. Taken together, these results demonstrate that the cidR gene product functions as a transcriptional activator of cidABC transcription in response to acetic acid accumulation in the growth medium.

Original languageEnglish (US)
Pages (from-to)5893-5900
Number of pages8
JournalJournal of bacteriology
Volume187
Issue number17
DOIs
StatePublished - Sep 1 2005

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Operon
N-Acetylmuramoyl-L-alanine Amidase
Staphylococcus aureus
Acetic Acid
Genes
Anti-Bacterial Agents
Growth
Northern Blotting
Bacteriophages
Proteins
Up-Regulation
Bacteria
Gene Expression
Glucose

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

A LysR-type regulator, CidR, is required for induction of the Staphylococcus aureus cidABC operon. / Yang, Soo Jin; Rice, Kelly C.; Brown, Raquel J.; Patton, Toni G.; Liou, Linda E.; Park, Yong Ho; Bayles, Kenneth W.

In: Journal of bacteriology, Vol. 187, No. 17, 01.09.2005, p. 5893-5900.

Research output: Contribution to journalArticle

Yang, Soo Jin ; Rice, Kelly C. ; Brown, Raquel J. ; Patton, Toni G. ; Liou, Linda E. ; Park, Yong Ho ; Bayles, Kenneth W. / A LysR-type regulator, CidR, is required for induction of the Staphylococcus aureus cidABC operon. In: Journal of bacteriology. 2005 ; Vol. 187, No. 17. pp. 5893-5900.
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