A highly adhesive and naturally derived sealant

Alexander Assmann, Andrea Vegh, Mohammad Ghasemi-Rad, Sara Bagherifard, George Cheng, Ehsan Shirzaei Sani, Guillermo U. Ruiz-Esparza, Iman Noshadi, Antonio D. Lassaletta, Sidhu Gangadharan, Ali Tamayol, Ali Khademhosseini, Nasim Annabi

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Conventional surgical techniques to seal and repair defects in highly stressed elastic tissues are insufficient. Therefore, this study aimed to engineer an inexpensive, highly adhesive, biocompatible, and biodegradable sealant based on a modified and naturally derived biopolymer, gelatin methacryloyl (GelMA). We tuned the degree of gelatin modification, prepolymer concentration, photoinitiator concentration, and crosslinking conditions to optimize the physical properties and adhesion of the photocrosslinked GelMA sealants. Following ASTM standard tests that target wound closure strength, shear resistance, and burst pressure, GelMA sealant was shown to exhibit adhesive properties that were superior to clinically used fibrin- and poly(ethylene glycol)-based glues. Chronic in vivo experiments in small as well as translational large animal models proved GelMA to effectively seal large lung leakages without the need for sutures or staples, presenting improved performance as compared to fibrin glue, poly(ethylene glycol) glue and sutures only. Furthermore, high biocompatibility of GelMA sealant was observed, as evidenced by a low inflammatory host response and fast in vivo degradation while allowing for adequate wound healing at the same time. Combining these results with the low costs, ease of synthesis and application of the material, GelMA sealant is envisioned to be commercialized not only as a sealant to stop air leakages, but also as a biocompatible and biodegradable hydrogel to support lung tissue regeneration.

Original languageEnglish (US)
Pages (from-to)115-127
Number of pages13
JournalBiomaterials
Volume140
DOIs
StatePublished - Sep 2017

Fingerprint

Sealants
Gelatin
Adhesives
Glues
Ethylene Glycol
Polyethylene glycols
Seals
Sutures
Tissue regeneration
Leakage (fluid)
Biopolymers
Shear Strength
Lung
Fibrin Tissue Adhesive
Elastic Tissue
Biocompatibility
Hydrogel
Hydrogels
Shear strength
Crosslinking

Keywords

  • Gelatin methacryloyl (GelMA)
  • Hydrogel
  • Lung lesion
  • Sealant
  • Wound repair

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Assmann, A., Vegh, A., Ghasemi-Rad, M., Bagherifard, S., Cheng, G., Sani, E. S., ... Annabi, N. (2017). A highly adhesive and naturally derived sealant. Biomaterials, 140, 115-127. https://doi.org/10.1016/j.biomaterials.2017.06.004

A highly adhesive and naturally derived sealant. / Assmann, Alexander; Vegh, Andrea; Ghasemi-Rad, Mohammad; Bagherifard, Sara; Cheng, George; Sani, Ehsan Shirzaei; Ruiz-Esparza, Guillermo U.; Noshadi, Iman; Lassaletta, Antonio D.; Gangadharan, Sidhu; Tamayol, Ali; Khademhosseini, Ali; Annabi, Nasim.

In: Biomaterials, Vol. 140, 09.2017, p. 115-127.

Research output: Contribution to journalArticle

Assmann, A, Vegh, A, Ghasemi-Rad, M, Bagherifard, S, Cheng, G, Sani, ES, Ruiz-Esparza, GU, Noshadi, I, Lassaletta, AD, Gangadharan, S, Tamayol, A, Khademhosseini, A & Annabi, N 2017, 'A highly adhesive and naturally derived sealant', Biomaterials, vol. 140, pp. 115-127. https://doi.org/10.1016/j.biomaterials.2017.06.004
Assmann A, Vegh A, Ghasemi-Rad M, Bagherifard S, Cheng G, Sani ES et al. A highly adhesive and naturally derived sealant. Biomaterials. 2017 Sep;140:115-127. https://doi.org/10.1016/j.biomaterials.2017.06.004
Assmann, Alexander ; Vegh, Andrea ; Ghasemi-Rad, Mohammad ; Bagherifard, Sara ; Cheng, George ; Sani, Ehsan Shirzaei ; Ruiz-Esparza, Guillermo U. ; Noshadi, Iman ; Lassaletta, Antonio D. ; Gangadharan, Sidhu ; Tamayol, Ali ; Khademhosseini, Ali ; Annabi, Nasim. / A highly adhesive and naturally derived sealant. In: Biomaterials. 2017 ; Vol. 140. pp. 115-127.
@article{d38e64e678c44b2983bd0a92af1cbfe1,
title = "A highly adhesive and naturally derived sealant",
abstract = "Conventional surgical techniques to seal and repair defects in highly stressed elastic tissues are insufficient. Therefore, this study aimed to engineer an inexpensive, highly adhesive, biocompatible, and biodegradable sealant based on a modified and naturally derived biopolymer, gelatin methacryloyl (GelMA). We tuned the degree of gelatin modification, prepolymer concentration, photoinitiator concentration, and crosslinking conditions to optimize the physical properties and adhesion of the photocrosslinked GelMA sealants. Following ASTM standard tests that target wound closure strength, shear resistance, and burst pressure, GelMA sealant was shown to exhibit adhesive properties that were superior to clinically used fibrin- and poly(ethylene glycol)-based glues. Chronic in vivo experiments in small as well as translational large animal models proved GelMA to effectively seal large lung leakages without the need for sutures or staples, presenting improved performance as compared to fibrin glue, poly(ethylene glycol) glue and sutures only. Furthermore, high biocompatibility of GelMA sealant was observed, as evidenced by a low inflammatory host response and fast in vivo degradation while allowing for adequate wound healing at the same time. Combining these results with the low costs, ease of synthesis and application of the material, GelMA sealant is envisioned to be commercialized not only as a sealant to stop air leakages, but also as a biocompatible and biodegradable hydrogel to support lung tissue regeneration.",
keywords = "Gelatin methacryloyl (GelMA), Hydrogel, Lung lesion, Sealant, Wound repair",
author = "Alexander Assmann and Andrea Vegh and Mohammad Ghasemi-Rad and Sara Bagherifard and George Cheng and Sani, {Ehsan Shirzaei} and Ruiz-Esparza, {Guillermo U.} and Iman Noshadi and Lassaletta, {Antonio D.} and Sidhu Gangadharan and Ali Tamayol and Ali Khademhosseini and Nasim Annabi",
year = "2017",
month = "9",
doi = "10.1016/j.biomaterials.2017.06.004",
language = "English (US)",
volume = "140",
pages = "115--127",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - A highly adhesive and naturally derived sealant

AU - Assmann, Alexander

AU - Vegh, Andrea

AU - Ghasemi-Rad, Mohammad

AU - Bagherifard, Sara

AU - Cheng, George

AU - Sani, Ehsan Shirzaei

AU - Ruiz-Esparza, Guillermo U.

AU - Noshadi, Iman

AU - Lassaletta, Antonio D.

AU - Gangadharan, Sidhu

AU - Tamayol, Ali

AU - Khademhosseini, Ali

AU - Annabi, Nasim

PY - 2017/9

Y1 - 2017/9

N2 - Conventional surgical techniques to seal and repair defects in highly stressed elastic tissues are insufficient. Therefore, this study aimed to engineer an inexpensive, highly adhesive, biocompatible, and biodegradable sealant based on a modified and naturally derived biopolymer, gelatin methacryloyl (GelMA). We tuned the degree of gelatin modification, prepolymer concentration, photoinitiator concentration, and crosslinking conditions to optimize the physical properties and adhesion of the photocrosslinked GelMA sealants. Following ASTM standard tests that target wound closure strength, shear resistance, and burst pressure, GelMA sealant was shown to exhibit adhesive properties that were superior to clinically used fibrin- and poly(ethylene glycol)-based glues. Chronic in vivo experiments in small as well as translational large animal models proved GelMA to effectively seal large lung leakages without the need for sutures or staples, presenting improved performance as compared to fibrin glue, poly(ethylene glycol) glue and sutures only. Furthermore, high biocompatibility of GelMA sealant was observed, as evidenced by a low inflammatory host response and fast in vivo degradation while allowing for adequate wound healing at the same time. Combining these results with the low costs, ease of synthesis and application of the material, GelMA sealant is envisioned to be commercialized not only as a sealant to stop air leakages, but also as a biocompatible and biodegradable hydrogel to support lung tissue regeneration.

AB - Conventional surgical techniques to seal and repair defects in highly stressed elastic tissues are insufficient. Therefore, this study aimed to engineer an inexpensive, highly adhesive, biocompatible, and biodegradable sealant based on a modified and naturally derived biopolymer, gelatin methacryloyl (GelMA). We tuned the degree of gelatin modification, prepolymer concentration, photoinitiator concentration, and crosslinking conditions to optimize the physical properties and adhesion of the photocrosslinked GelMA sealants. Following ASTM standard tests that target wound closure strength, shear resistance, and burst pressure, GelMA sealant was shown to exhibit adhesive properties that were superior to clinically used fibrin- and poly(ethylene glycol)-based glues. Chronic in vivo experiments in small as well as translational large animal models proved GelMA to effectively seal large lung leakages without the need for sutures or staples, presenting improved performance as compared to fibrin glue, poly(ethylene glycol) glue and sutures only. Furthermore, high biocompatibility of GelMA sealant was observed, as evidenced by a low inflammatory host response and fast in vivo degradation while allowing for adequate wound healing at the same time. Combining these results with the low costs, ease of synthesis and application of the material, GelMA sealant is envisioned to be commercialized not only as a sealant to stop air leakages, but also as a biocompatible and biodegradable hydrogel to support lung tissue regeneration.

KW - Gelatin methacryloyl (GelMA)

KW - Hydrogel

KW - Lung lesion

KW - Sealant

KW - Wound repair

UR - http://www.scopus.com/inward/record.url?scp=85021071764&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85021071764&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2017.06.004

DO - 10.1016/j.biomaterials.2017.06.004

M3 - Article

C2 - 28646685

AN - SCOPUS:85021071764

VL - 140

SP - 115

EP - 127

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

ER -