A Hg(II) INDUCED CONFORMATIONAL CHANGE IN PENICILLINASE

Leo D'Souza, Madaiah Madaiah, Richard A. Day, Kenneth Nickerson

Research output: Contribution to journalArticle

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Abstract

Penicillinase (E.C.3.5.2.6) from Bacillus cereus 569/H is inhibited by Hg(II). The inhibition is characterized by non‐competitive kinetics and can be reversed by EDTA. A Hg(II) induced conformational change is indicated because: (1) The EDTA regenerated activity is unstable and is rapidly converted to an iodine‐sensitive state, and (2) An irreversible change in the circular dichroism spectrum at 222 nm is found.

Original languageEnglish (US)
Pages (from-to)251-259
Number of pages9
JournalInternational Journal of Peptide and Protein Research
Volume7
Issue number3
DOIs
StatePublished - Jan 1 1975

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Penicillinase
Edetic Acid
Bacillus cereus
Circular Dichroism
Kinetics

ASJC Scopus subject areas

  • Biochemistry

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A Hg(II) INDUCED CONFORMATIONAL CHANGE IN PENICILLINASE. / D'Souza, Leo; Madaiah, Madaiah; Day, Richard A.; Nickerson, Kenneth.

In: International Journal of Peptide and Protein Research, Vol. 7, No. 3, 01.01.1975, p. 251-259.

Research output: Contribution to journalArticle

D'Souza, Leo ; Madaiah, Madaiah ; Day, Richard A. ; Nickerson, Kenneth. / A Hg(II) INDUCED CONFORMATIONAL CHANGE IN PENICILLINASE. In: International Journal of Peptide and Protein Research. 1975 ; Vol. 7, No. 3. pp. 251-259.
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