A genetically encoded fluorescent probe in mammalian cells

Abhishek Chatterjee, Jiantao Guo, Hyun Soo Lee, Peter G. Schultz

Research output: Contribution to journalArticle

87 Scopus citations

Abstract

Fluorescent reporters are useful in vitro and in vivo probes of protein structure, function, and localization. Here we report that the fluorescent amino acid, 3-(6-acetylnaphthalen-2-ylamino)-2-aminopropanoic acid (Anap), can be site-specifically incorporated into proteins in mammalian cells in response to the TAG codon with high efficiency using an orthogonal amber suppressor tRNA/aminoacyl-tRNA synthetase (aaRS) pair. We further demonstrate that Anap can be used to image the subcellular localization of proteins in live mammalian cells. The small size of Anap, its environment-sensitive fluorescence, and the ability to introduce Anap at specific sites in the proteome by simple mutagenesis make it a unique and valuable tool in eukaryotic cell biology.

Original languageEnglish (US)
Pages (from-to)12540-12543
Number of pages4
JournalJournal of the American Chemical Society
Volume135
Issue number34
DOIs
StatePublished - Aug 28 2013

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ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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