A genetically encoded cyclobutene probe for labelling of live cells

K. Liu, B. Enns, B. Evans, N. Wang, X. Shang, W. Sittiwong, P. H. Dussault, J. Guo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have identified an aminoacyl-tRNA synthetase/tRNA pair for the efficient and site-specific incorporation of a cyclobutene-containing amino acid into proteins in response to an amber nonsense codon. Fast and fluorescent labeling of purified proteins and intact proteins in live cells was demonstrated using the inverse electron demand Diels-Alder reaction with a tetrazine.

Original languageEnglish (US)
Pages (from-to)10604-10607
Number of pages4
JournalChemical Communications
Volume53
Issue number76
DOIs
StatePublished - Jan 1 2017

Fingerprint

Labeling
Proteins
Amber
Amino Acyl-tRNA Synthetases
Transfer RNA
Amino acids
Amino Acids
Electrons

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

Liu, K., Enns, B., Evans, B., Wang, N., Shang, X., Sittiwong, W., ... Guo, J. (2017). A genetically encoded cyclobutene probe for labelling of live cells. Chemical Communications, 53(76), 10604-10607. https://doi.org/10.1039/c7cc05580c

A genetically encoded cyclobutene probe for labelling of live cells. / Liu, K.; Enns, B.; Evans, B.; Wang, N.; Shang, X.; Sittiwong, W.; Dussault, P. H.; Guo, J.

In: Chemical Communications, Vol. 53, No. 76, 01.01.2017, p. 10604-10607.

Research output: Contribution to journalArticle

Liu, K, Enns, B, Evans, B, Wang, N, Shang, X, Sittiwong, W, Dussault, PH & Guo, J 2017, 'A genetically encoded cyclobutene probe for labelling of live cells', Chemical Communications, vol. 53, no. 76, pp. 10604-10607. https://doi.org/10.1039/c7cc05580c
Liu K, Enns B, Evans B, Wang N, Shang X, Sittiwong W et al. A genetically encoded cyclobutene probe for labelling of live cells. Chemical Communications. 2017 Jan 1;53(76):10604-10607. https://doi.org/10.1039/c7cc05580c
Liu, K. ; Enns, B. ; Evans, B. ; Wang, N. ; Shang, X. ; Sittiwong, W. ; Dussault, P. H. ; Guo, J. / A genetically encoded cyclobutene probe for labelling of live cells. In: Chemical Communications. 2017 ; Vol. 53, No. 76. pp. 10604-10607.
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