A fratricidal mechanism is responsible for eDNA release and contributes to biofilm development of Enterococcus faecalis

Vinai Chittezham Thomas, Yasuaki Hiromasa, Nathan Harms, Lance Thurlow, John Tomich, Lynn E. Hancock

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

Extracellular DNA (eDNA), a by-product of cell lysis, was recently established as a critical structural component of the Enterococcus faecalis biofilm matrix. Here, we describe fratricide as the governing principle behind gelatinase (GelE)-mediated cell death and eDNA release. GFP reporter assays confirmed that GBAP (gelatinase biosynthesis-activating pheromone) quorum non-responders (GelE-SprE-) were a minority subpopulation of prey cells susceptible to the targeted fratricidal action of the quorum responsive predatorial majority (GelE+SprE+). The killing action is dependent on GelE, and the GelE producer population is protected from self-destruction by the co-production of SprE as an immunity protein. Targeted gene inactivation and protein interaction studies demonstrate that extracellular proteases execute their characteristic effects following downstream interactions with the primary autolysin, AtlA. Finally, we address a mechanism by which GelE and SprE may modify the cell wall affinity of proteolytically processed AtlA resulting in either a pro- or anti-lytic outcome.

Original languageEnglish (US)
Pages (from-to)1022-1036
Number of pages15
JournalMolecular Microbiology
Volume72
Issue number4
DOIs
StatePublished - May 1 2009

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Gelatinases
Enterococcus faecalis
Biofilms
DNA
N-Acetylmuramoyl-L-alanine Amidase
Gene Silencing
Cell Wall
Immunity
Proteins
Peptide Hydrolases
Cell Death
Population

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

A fratricidal mechanism is responsible for eDNA release and contributes to biofilm development of Enterococcus faecalis. / Thomas, Vinai Chittezham; Hiromasa, Yasuaki; Harms, Nathan; Thurlow, Lance; Tomich, John; Hancock, Lynn E.

In: Molecular Microbiology, Vol. 72, No. 4, 01.05.2009, p. 1022-1036.

Research output: Contribution to journalArticle

Thomas, Vinai Chittezham ; Hiromasa, Yasuaki ; Harms, Nathan ; Thurlow, Lance ; Tomich, John ; Hancock, Lynn E. / A fratricidal mechanism is responsible for eDNA release and contributes to biofilm development of Enterococcus faecalis. In: Molecular Microbiology. 2009 ; Vol. 72, No. 4. pp. 1022-1036.
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