A four-season longitudinal study of enterohaemorrhagic Escherichia coli in beef cow–calf herds in Mississippi and Nebraska

L. G. Schneider, G. L. Lewis, R. A. Moxley, D. R. Smith

Research output: Contribution to journalArticle

Abstract

Our objective was to describe the probability of detecting seven serogroups of enterohaemorrhagic Escherichia coli (EHEC-7) of public health importance in faecal samples from beef cow–calf herds and to test for factors associated with their detection. Fresh faecal samples (n = 85) from two Mississippi and two Nebraska herds were collected in each of four seasons. Samples were tested for each EHEC-7 serogroup by a molecular screening assay. Separate management groups within herds were sampled, and group-level factors were recorded. To measure the effects of factors on faecal shedding of EHEC-7, separate multivariable logistic regression models were used, accounting for the random effect of clustering by group within farm. Statistical significance was set α = 0.05. Fifty-nine samples (4.3%) were positive for EHEC O26, and Nebraska samples were more likely to be positive than Mississippi samples (OR = 12.4, 95% CI: 1.1, 139.2). Forty-four samples (3.2%) were positive for EHEC O45. Odds for detection were greater in the summer than all other seasons combined (OR = 4.2, 95% CI: 1.3, 14.0), and odds decreased if a precipitation event occurred (OR = 0.07, 95% CI: 0.006, 0.8). EHEC O103 was detected in 66 samples (4.9%) with increased probability to be detected at increased temperature. EHEC O111 was detected in 71 samples (5.2%), and 43 samples (3.2%) were positive for EHEC O145. Both EHEC O111 and O145 were associated separately with season, with greater probability for detection in the summer. Eighteen (1.3%) and 68 (5.0%) samples were positive for EHEC O121 and EHEC O157, respectively. We failed to detect significant explanatory factors associated with probability to detect EHEC O121 or O157. Factors that vary by time and place, such as precipitation, ambient temperature, region and season, are uniquely associated with the probability to detect EHEC-7 in fresh faeces collected from cow–calf herds.

Original languageEnglish (US)
Pages (from-to)552-559
Number of pages8
JournalZoonoses and Public Health
Volume65
Issue number5
DOIs
StatePublished - Aug 2018

Fingerprint

Enterohemorrhagic Escherichia coli
Mississippi
enterohemorrhagic Escherichia coli
longitudinal studies
Longitudinal Studies
beef
herds
sampling
serotypes
Red Meat
Logistic Models
summer
Temperature
ambient temperature
public health
Feces
feces

Keywords

  • Escherichia coli spp.
  • cattle
  • cow–calf
  • enterohaemorrhagic Escherichia coli
  • epidemiology
  • foodborne pathogens

ASJC Scopus subject areas

  • Epidemiology
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

A four-season longitudinal study of enterohaemorrhagic Escherichia coli in beef cow–calf herds in Mississippi and Nebraska. / Schneider, L. G.; Lewis, G. L.; Moxley, R. A.; Smith, D. R.

In: Zoonoses and Public Health, Vol. 65, No. 5, 08.2018, p. 552-559.

Research output: Contribution to journalArticle

@article{a33b1afd3405493bbe6867210a166cf4,
title = "A four-season longitudinal study of enterohaemorrhagic Escherichia coli in beef cow–calf herds in Mississippi and Nebraska",
abstract = "Our objective was to describe the probability of detecting seven serogroups of enterohaemorrhagic Escherichia coli (EHEC-7) of public health importance in faecal samples from beef cow–calf herds and to test for factors associated with their detection. Fresh faecal samples (n = 85) from two Mississippi and two Nebraska herds were collected in each of four seasons. Samples were tested for each EHEC-7 serogroup by a molecular screening assay. Separate management groups within herds were sampled, and group-level factors were recorded. To measure the effects of factors on faecal shedding of EHEC-7, separate multivariable logistic regression models were used, accounting for the random effect of clustering by group within farm. Statistical significance was set α = 0.05. Fifty-nine samples (4.3{\%}) were positive for EHEC O26, and Nebraska samples were more likely to be positive than Mississippi samples (OR = 12.4, 95{\%} CI: 1.1, 139.2). Forty-four samples (3.2{\%}) were positive for EHEC O45. Odds for detection were greater in the summer than all other seasons combined (OR = 4.2, 95{\%} CI: 1.3, 14.0), and odds decreased if a precipitation event occurred (OR = 0.07, 95{\%} CI: 0.006, 0.8). EHEC O103 was detected in 66 samples (4.9{\%}) with increased probability to be detected at increased temperature. EHEC O111 was detected in 71 samples (5.2{\%}), and 43 samples (3.2{\%}) were positive for EHEC O145. Both EHEC O111 and O145 were associated separately with season, with greater probability for detection in the summer. Eighteen (1.3{\%}) and 68 (5.0{\%}) samples were positive for EHEC O121 and EHEC O157, respectively. We failed to detect significant explanatory factors associated with probability to detect EHEC O121 or O157. Factors that vary by time and place, such as precipitation, ambient temperature, region and season, are uniquely associated with the probability to detect EHEC-7 in fresh faeces collected from cow–calf herds.",
keywords = "Escherichia coli spp., cattle, cow–calf, enterohaemorrhagic Escherichia coli, epidemiology, foodborne pathogens",
author = "Schneider, {L. G.} and Lewis, {G. L.} and Moxley, {R. A.} and Smith, {D. R.}",
year = "2018",
month = "8",
doi = "10.1111/zph.12465",
language = "English (US)",
volume = "65",
pages = "552--559",
journal = "Zoonoses and Public Health",
issn = "1863-1959",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "5",

}

TY - JOUR

T1 - A four-season longitudinal study of enterohaemorrhagic Escherichia coli in beef cow–calf herds in Mississippi and Nebraska

AU - Schneider, L. G.

AU - Lewis, G. L.

AU - Moxley, R. A.

AU - Smith, D. R.

PY - 2018/8

Y1 - 2018/8

N2 - Our objective was to describe the probability of detecting seven serogroups of enterohaemorrhagic Escherichia coli (EHEC-7) of public health importance in faecal samples from beef cow–calf herds and to test for factors associated with their detection. Fresh faecal samples (n = 85) from two Mississippi and two Nebraska herds were collected in each of four seasons. Samples were tested for each EHEC-7 serogroup by a molecular screening assay. Separate management groups within herds were sampled, and group-level factors were recorded. To measure the effects of factors on faecal shedding of EHEC-7, separate multivariable logistic regression models were used, accounting for the random effect of clustering by group within farm. Statistical significance was set α = 0.05. Fifty-nine samples (4.3%) were positive for EHEC O26, and Nebraska samples were more likely to be positive than Mississippi samples (OR = 12.4, 95% CI: 1.1, 139.2). Forty-four samples (3.2%) were positive for EHEC O45. Odds for detection were greater in the summer than all other seasons combined (OR = 4.2, 95% CI: 1.3, 14.0), and odds decreased if a precipitation event occurred (OR = 0.07, 95% CI: 0.006, 0.8). EHEC O103 was detected in 66 samples (4.9%) with increased probability to be detected at increased temperature. EHEC O111 was detected in 71 samples (5.2%), and 43 samples (3.2%) were positive for EHEC O145. Both EHEC O111 and O145 were associated separately with season, with greater probability for detection in the summer. Eighteen (1.3%) and 68 (5.0%) samples were positive for EHEC O121 and EHEC O157, respectively. We failed to detect significant explanatory factors associated with probability to detect EHEC O121 or O157. Factors that vary by time and place, such as precipitation, ambient temperature, region and season, are uniquely associated with the probability to detect EHEC-7 in fresh faeces collected from cow–calf herds.

AB - Our objective was to describe the probability of detecting seven serogroups of enterohaemorrhagic Escherichia coli (EHEC-7) of public health importance in faecal samples from beef cow–calf herds and to test for factors associated with their detection. Fresh faecal samples (n = 85) from two Mississippi and two Nebraska herds were collected in each of four seasons. Samples were tested for each EHEC-7 serogroup by a molecular screening assay. Separate management groups within herds were sampled, and group-level factors were recorded. To measure the effects of factors on faecal shedding of EHEC-7, separate multivariable logistic regression models were used, accounting for the random effect of clustering by group within farm. Statistical significance was set α = 0.05. Fifty-nine samples (4.3%) were positive for EHEC O26, and Nebraska samples were more likely to be positive than Mississippi samples (OR = 12.4, 95% CI: 1.1, 139.2). Forty-four samples (3.2%) were positive for EHEC O45. Odds for detection were greater in the summer than all other seasons combined (OR = 4.2, 95% CI: 1.3, 14.0), and odds decreased if a precipitation event occurred (OR = 0.07, 95% CI: 0.006, 0.8). EHEC O103 was detected in 66 samples (4.9%) with increased probability to be detected at increased temperature. EHEC O111 was detected in 71 samples (5.2%), and 43 samples (3.2%) were positive for EHEC O145. Both EHEC O111 and O145 were associated separately with season, with greater probability for detection in the summer. Eighteen (1.3%) and 68 (5.0%) samples were positive for EHEC O121 and EHEC O157, respectively. We failed to detect significant explanatory factors associated with probability to detect EHEC O121 or O157. Factors that vary by time and place, such as precipitation, ambient temperature, region and season, are uniquely associated with the probability to detect EHEC-7 in fresh faeces collected from cow–calf herds.

KW - Escherichia coli spp.

KW - cattle

KW - cow–calf

KW - enterohaemorrhagic Escherichia coli

KW - epidemiology

KW - foodborne pathogens

UR - http://www.scopus.com/inward/record.url?scp=85044301920&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85044301920&partnerID=8YFLogxK

U2 - 10.1111/zph.12465

DO - 10.1111/zph.12465

M3 - Article

C2 - 29573177

AN - SCOPUS:85044301920

VL - 65

SP - 552

EP - 559

JO - Zoonoses and Public Health

JF - Zoonoses and Public Health

SN - 1863-1959

IS - 5

ER -