A family of human β3-galactosyltransferases: Characterization of four members of a UDP-galactose:β-N-acetyl-glucosamine/β-N-acetyl-galactosamine β-1,3-galactosyltransferase family

Margarida Amado, Raquel Almeida, Fatima Carneiro, Steven B. Levery, Eric H. Holmes, Mitsuharu Nomoto, Michael A Hollingsworth, Helle Hassan, Tilo Schwientek, Peter A. Nielsen, Eric P. Bennett, Henrik Clausen

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

BLAST analysis of expressed sequence tags (ESTs) using the coding sequence of a human UDP-galactose:β-N-acetyl-glucosamine β-1,3- galactosyltransferase, designated β3Gal-T1, revealed no ESTs with identical sequences but a large number with similarity. Three different sets of overlapping ESTs with sequence similarities to β3Gal-T1 were compiled, and complete coding regions of these genes were obtained. Expression of two of these genes in the Baculo virus system showed that one represented a UDP- galactose:β-N-acetyl-glucosemine β-1,3-galactosyltransferase (β3Gal-T2) with similar kinetic properties as β3Gal-T1. Another gene represented a UDP- galactose:β-N-acetyl-galactosamine β-1,3-galactosyltransferase (β3Gal-T4) involved in G(M1)/G(D1) ganglioside synthesis, and this gene was highly similar to a recently reported rat G(D1) synthase (Miyazaki, H., Fukumoto, S., Okada, M., Hasegawa, T., and Furukawa, K. (1997) J. Biol. Chem. 272, 24794-24799). Northern analysis of mRNA from human organs with the four homologous cDNA revealed different expression patterns. β3Gal-T1 mRNA was expressed in brain, β3Gal-T2 was expressed in brain and heart, and β3Gal- T3 and -T4 were more widely expressed. The coding regions for each of the four genes were contained in single exons. β3Gal-T2, -T3, and -T4 were localized to 1q31, 3q25, and 6p21.3, respectively, by EST mapping. The results demonstrate the existence of a family of homologous β3- galactosyltransferase genes.

Original languageEnglish (US)
Pages (from-to)12770-12778
Number of pages9
JournalJournal of Biological Chemistry
Volume273
Issue number21
DOIs
StatePublished - May 22 1998

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Uridine Diphosphate Galactose
Galactosyltransferases
Galactosamine
Glucosamine
Expressed Sequence Tags
Genes
Brain
G(M1) Ganglioside
Messenger RNA
Gangliosides
Exons
Viruses
Complementary DNA
Rats
Gene Expression
Kinetics

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

A family of human β3-galactosyltransferases : Characterization of four members of a UDP-galactose:β-N-acetyl-glucosamine/β-N-acetyl-galactosamine β-1,3-galactosyltransferase family. / Amado, Margarida; Almeida, Raquel; Carneiro, Fatima; Levery, Steven B.; Holmes, Eric H.; Nomoto, Mitsuharu; Hollingsworth, Michael A; Hassan, Helle; Schwientek, Tilo; Nielsen, Peter A.; Bennett, Eric P.; Clausen, Henrik.

In: Journal of Biological Chemistry, Vol. 273, No. 21, 22.05.1998, p. 12770-12778.

Research output: Contribution to journalArticle

Amado, Margarida ; Almeida, Raquel ; Carneiro, Fatima ; Levery, Steven B. ; Holmes, Eric H. ; Nomoto, Mitsuharu ; Hollingsworth, Michael A ; Hassan, Helle ; Schwientek, Tilo ; Nielsen, Peter A. ; Bennett, Eric P. ; Clausen, Henrik. / A family of human β3-galactosyltransferases : Characterization of four members of a UDP-galactose:β-N-acetyl-glucosamine/β-N-acetyl-galactosamine β-1,3-galactosyltransferase family. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 21. pp. 12770-12778.
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abstract = "BLAST analysis of expressed sequence tags (ESTs) using the coding sequence of a human UDP-galactose:β-N-acetyl-glucosamine β-1,3- galactosyltransferase, designated β3Gal-T1, revealed no ESTs with identical sequences but a large number with similarity. Three different sets of overlapping ESTs with sequence similarities to β3Gal-T1 were compiled, and complete coding regions of these genes were obtained. Expression of two of these genes in the Baculo virus system showed that one represented a UDP- galactose:β-N-acetyl-glucosemine β-1,3-galactosyltransferase (β3Gal-T2) with similar kinetic properties as β3Gal-T1. Another gene represented a UDP- galactose:β-N-acetyl-galactosamine β-1,3-galactosyltransferase (β3Gal-T4) involved in G(M1)/G(D1) ganglioside synthesis, and this gene was highly similar to a recently reported rat G(D1) synthase (Miyazaki, H., Fukumoto, S., Okada, M., Hasegawa, T., and Furukawa, K. (1997) J. Biol. Chem. 272, 24794-24799). Northern analysis of mRNA from human organs with the four homologous cDNA revealed different expression patterns. β3Gal-T1 mRNA was expressed in brain, β3Gal-T2 was expressed in brain and heart, and β3Gal- T3 and -T4 were more widely expressed. The coding regions for each of the four genes were contained in single exons. β3Gal-T2, -T3, and -T4 were localized to 1q31, 3q25, and 6p21.3, respectively, by EST mapping. The results demonstrate the existence of a family of homologous β3- galactosyltransferase genes.",
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T2 - Characterization of four members of a UDP-galactose:β-N-acetyl-glucosamine/β-N-acetyl-galactosamine β-1,3-galactosyltransferase family

AU - Amado, Margarida

AU - Almeida, Raquel

AU - Carneiro, Fatima

AU - Levery, Steven B.

AU - Holmes, Eric H.

AU - Nomoto, Mitsuharu

AU - Hollingsworth, Michael A

AU - Hassan, Helle

AU - Schwientek, Tilo

AU - Nielsen, Peter A.

AU - Bennett, Eric P.

AU - Clausen, Henrik

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