A facile system for encoding unnatural amino acids in mammalian cells

Peng R. Chen, Dan Groff, Jiantao Guo, Weijia Ou, Susan Cellitti, Bernhard H. Geierstanger, Peter G. Schultz

Research output: Contribution to journalArticle

173 Citations (Scopus)

Abstract

A shuttle system has been developed to genetically encode unnatural amino acids in mammalian cells using aminoacyl-tRNA synthetases (aaRSs) evolved in E. coli. A pyrrolysyl-tRNA synthetase (PylRS) mutant was evolved in E. coli that selectively aminoacylates a cognate nonsense suppressor tRNA with a photocaged lysine derivative. Transfer of this orthogonal tRNA-aaRS pair into mammalian cells made possible the selective incorporation of this unnatural amino acid into proteins.

Original languageEnglish (US)
Pages (from-to)4052-4055
Number of pages4
JournalAngewandte Chemie - International Edition
Volume48
Issue number22
DOIs
StatePublished - May 18 2009

Fingerprint

Amino Acyl-tRNA Synthetases
Transfer RNA
Escherichia coli
Amino acids
Cells
Amino Acids
Lysine
Derivatives
Proteins

Keywords

  • Gene expression
  • Konnatural amino acids
  • Photocaged lysine
  • tRNA

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Cite this

Chen, P. R., Groff, D., Guo, J., Ou, W., Cellitti, S., Geierstanger, B. H., & Schultz, P. G. (2009). A facile system for encoding unnatural amino acids in mammalian cells. Angewandte Chemie - International Edition, 48(22), 4052-4055. https://doi.org/10.1002/anie.200900683

A facile system for encoding unnatural amino acids in mammalian cells. / Chen, Peng R.; Groff, Dan; Guo, Jiantao; Ou, Weijia; Cellitti, Susan; Geierstanger, Bernhard H.; Schultz, Peter G.

In: Angewandte Chemie - International Edition, Vol. 48, No. 22, 18.05.2009, p. 4052-4055.

Research output: Contribution to journalArticle

Chen, PR, Groff, D, Guo, J, Ou, W, Cellitti, S, Geierstanger, BH & Schultz, PG 2009, 'A facile system for encoding unnatural amino acids in mammalian cells', Angewandte Chemie - International Edition, vol. 48, no. 22, pp. 4052-4055. https://doi.org/10.1002/anie.200900683
Chen, Peng R. ; Groff, Dan ; Guo, Jiantao ; Ou, Weijia ; Cellitti, Susan ; Geierstanger, Bernhard H. ; Schultz, Peter G. / A facile system for encoding unnatural amino acids in mammalian cells. In: Angewandte Chemie - International Edition. 2009 ; Vol. 48, No. 22. pp. 4052-4055.
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