A dot enzyme immunoassay for detection of IgM antibodies against phenolic glycolipid-I sera from leprosy patients

S. Kumar, K. D. Moudgil, A. H. Band, P. R. Naraynan, S. K. Gupta, A. K. Sharma, G. P. Talwar

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A visual dipstick dot enzyme immunoassay (EIA) for diagnosis of leprosy is described. The assay is based on detection of IgM antibodies against phenolic glycolipid (PGL-I) in sera from leprosy patients. The antigen (PGL-I or synthetic disaccharide of PGL-I) was dotted on a nitrocellulose pad stuck on a plastic strip (dipstick). Sera were used at a dilution of 1:200. Peroxidase coupled mouse anti-human IgM monoclonal antibodies were used as the conjugate. A positive test gave a blue dot against a white background. The test was highly specific for leprosy, and was quite sensitive for detection of bacilliferous (BL/LL) leprosy. The antigen dotted and preblocked dipsticks stored at room temperature upto 4 months of observation period, were unable in the assay.

Original languageEnglish (US)
Pages (from-to)185-190
Number of pages6
JournalIndian Journal of Leprosy
Volume58
Issue number2
StatePublished - Dec 1 1986

Fingerprint

Glycolipids
Leprosy
Immunoenzyme Techniques
Immunoglobulin M
Antibodies
Serum
Antigens
Collodion
Disaccharides
Peroxidase
Plastics
Monoclonal Antibodies
Observation
Temperature

ASJC Scopus subject areas

  • Dermatology
  • Infectious Diseases

Cite this

Kumar, S., Moudgil, K. D., Band, A. H., Naraynan, P. R., Gupta, S. K., Sharma, A. K., & Talwar, G. P. (1986). A dot enzyme immunoassay for detection of IgM antibodies against phenolic glycolipid-I sera from leprosy patients. Indian Journal of Leprosy, 58(2), 185-190.

A dot enzyme immunoassay for detection of IgM antibodies against phenolic glycolipid-I sera from leprosy patients. / Kumar, S.; Moudgil, K. D.; Band, A. H.; Naraynan, P. R.; Gupta, S. K.; Sharma, A. K.; Talwar, G. P.

In: Indian Journal of Leprosy, Vol. 58, No. 2, 01.12.1986, p. 185-190.

Research output: Contribution to journalArticle

Kumar, S, Moudgil, KD, Band, AH, Naraynan, PR, Gupta, SK, Sharma, AK & Talwar, GP 1986, 'A dot enzyme immunoassay for detection of IgM antibodies against phenolic glycolipid-I sera from leprosy patients', Indian Journal of Leprosy, vol. 58, no. 2, pp. 185-190.
Kumar, S. ; Moudgil, K. D. ; Band, A. H. ; Naraynan, P. R. ; Gupta, S. K. ; Sharma, A. K. ; Talwar, G. P. / A dot enzyme immunoassay for detection of IgM antibodies against phenolic glycolipid-I sera from leprosy patients. In: Indian Journal of Leprosy. 1986 ; Vol. 58, No. 2. pp. 185-190.
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