A dose dependent impact of prebiotic galactooligosaccharides on the intestinal microbiota of healthy adults

L. M.G. Davis, I. Martínez, J. Walter, R. Hutkins

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

The goal of this research was to determine the effect of different doses of galactooligosaccharide (GOS) on the fecal microbiota of healthy adults, with a focus on bifidobacteria. The study was designed as a single-blinded study, with eighteen subjects consuming GOS-containing chocolate chews at four increasing dosage levels; 0, 2.5, 5.0, and 10.0 g. Subjects consumed each dose for 3 weeks, with a two-week baseline period preceding the study and a two-week washout period at the end. Fecal samples were collected weekly and analyzed by cultural and molecular methods. Cultural methods were used for bifidobacteria, Bacteroides, enterobacteria, enterococci, lactobacilli, and total anaerobes; culture-independent methods included denaturing gradient gel electrophoresis (DGGE) and quantitative real-time PCR (qRT-PCR) using Bifidobacterium-specific primers. All three methods revealed an increase in bifidobacteria populations, as the GOS dosage increased to 5 or 10 g. Enumeration of bifidobacteria by qRT-PCR showed a high inter-subject variation in bifidogenic effect and indicated a subset of 9 GOS responders among the eighteen subjects. There were no differences, however, in the initial levels of bifidobacteria between the responding individuals and the non-responding individuals. Collectively, this study showed that a high purity GOS, administered in a confection product at doses of 5 g or higher, was bifidogenic, while a dose of 2.5 g showed no significant effect. However, the results also showed that even when GOS was administered for many weeks and at high doses, there were still some individuals for which a bifidogenic response did not occur.

Original languageEnglish (US)
Pages (from-to)285-292
Number of pages8
JournalInternational Journal of Food Microbiology
Volume144
Issue number2
DOIs
StatePublished - Dec 15 2010

Fingerprint

galactooligosaccharides
Prebiotics
Bifidobacterium
prebiotics
Electrophoresis
intestinal microorganisms
Gels
dosage
Real-Time Polymerase Chain Reaction
Candy
quantitative polymerase chain reaction
Denaturing Gradient Gel Electrophoresis
Bacteroides
Microbiota
Enterococcus
Lactobacillus
Enterobacteriaceae
anaerobes
chocolate
denaturing gradient gel electrophoresis

Keywords

  • Bifidobacterium
  • Galactooligosaccharides
  • Intestinal microbiota
  • Prebiotics

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Cite this

A dose dependent impact of prebiotic galactooligosaccharides on the intestinal microbiota of healthy adults. / Davis, L. M.G.; Martínez, I.; Walter, J.; Hutkins, R.

In: International Journal of Food Microbiology, Vol. 144, No. 2, 15.12.2010, p. 285-292.

Research output: Contribution to journalArticle

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