A diversity of Antibiotic-resistant Staphylococcus spp. in a Public Transportation System

Pamela J. Yeh, Dawn M. Simon, Jess A. Millar, H. Forrest Alexander, Darleen Franklin

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objectives: Our goal was to determine the diversity and abundance of Staphylococcus bacteria on different components of a public transportation system in a mid-sized US city (Portland, Oregon) and to examine the level of drug resistance in these bacteria. Methods: We collected 70 samples from 2 cm × 4 cm sections from seven different areas on buses and trains in Portland, USA, taking 10 samples from each area. We isolated a subset of 14 suspected Staphylococcus spp. colonies based on phenotype, and constructed a phylogeny from16S rRNA sequences to assist in identification. We used the Kirby-Bauer disk diffusion method to determine resistance levels to six common antibiotics. Results: We found a range of pathogenic Staphylococcus species. The mean bacterial colony counts were 97.1 on bus and train floors, 80.1 in cloth seats, 9.5 on handrails, 8.6 on seats and armrests at bus stops, 3.8 on the underside of seats, 2.2 on windows, and 1.8 on vinyl seats per 8 cm2 sample area. These differences were significant (p < 0.001). Of the 14 isolates sequenced, 11 were staphylococci, and of these, five were resistant to penicillin and ampicillin, while only two displayed intermediate resistance to bacitracin. All 11 isolates were sensitive to trimethoprim-sulfamethoxazole, vancomycin, and tetracycline. Conclusions: We found six different strains of Staphylococcus, and while there were varying levels of drug resistance, we did not find extensive levels of multidrug-resistant bacteria, and no S. aureus was found. We found floors and cloth seats to be areas on buses and trains that showed particularly high levels of bacteria.

Original languageEnglish (US)
Pages (from-to)202-209
Number of pages8
JournalOsong Public Health and Research Perspectives
Volume2
Issue number3
DOIs
StatePublished - Dec 1 2011

Fingerprint

Staphylococcus
Motor Vehicles
Anti-Bacterial Agents
Bacteria
Drug Resistance
Disk Diffusion Antimicrobial Tests
Bacitracin
Bacterial Load
Sulfamethoxazole Drug Combination Trimethoprim
Vancomycin
Ampicillin
Phylogeny
Tetracycline
Penicillins
Phenotype

Keywords

  • Antibiotic resistance
  • Community-acquired infections
  • Environmental microbiology
  • Methicillin-resistant Staphylococcus aureus
  • Pathogen transmission
  • Staphylococcus
  • Transportation

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

A diversity of Antibiotic-resistant Staphylococcus spp. in a Public Transportation System. / Yeh, Pamela J.; Simon, Dawn M.; Millar, Jess A.; Alexander, H. Forrest; Franklin, Darleen.

In: Osong Public Health and Research Perspectives, Vol. 2, No. 3, 01.12.2011, p. 202-209.

Research output: Contribution to journalArticle

Yeh, Pamela J. ; Simon, Dawn M. ; Millar, Jess A. ; Alexander, H. Forrest ; Franklin, Darleen. / A diversity of Antibiotic-resistant Staphylococcus spp. in a Public Transportation System. In: Osong Public Health and Research Perspectives. 2011 ; Vol. 2, No. 3. pp. 202-209.
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