A cytoplasm-specific activity encoded by the Trithorax-like ATX1 gene

Ivan Ndamukong, Hanna Lapko, Ronald Cerny, Zoya V Avramova

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Eukaryotes produce multiple products from a single gene locus by alternative splicing, translation or promoter usage as mechanisms expanding the complexity of their proteome. Trithorax proteins, including the Arabidopsis Trithorax-like protein ATX1, are histone modifiers regulating gene activity. Here, we report that a novel member of the Trithorax family has a role unrelated to chromatin. It is encoded from an internal promoter in the ATX1 locus as an isoform containing only the SET domain (soloSET). It is located exclusively in the cytoplasm and its substrate is the elongation factor 1A (EF1A). Loss of SET, but not of the histone modifying ATX1-SET activity, affects cytoskeletal actin bundling illustrating that the two isoforms have distinct functions in Arabidopsis cells.

Original languageEnglish (US)
Pages (from-to)4709-4718
Number of pages10
JournalNucleic acids research
Volume39
Issue number11
DOIs
StatePublished - Jun 1 2011

Fingerprint

Histones
Protein Isoforms
Cytoplasm
Modifier Genes
Arabidopsis Proteins
Peptide Elongation Factors
Alternative Splicing
Proteome
Eukaryota
Arabidopsis
Genes
Chromatin
Actins
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

A cytoplasm-specific activity encoded by the Trithorax-like ATX1 gene. / Ndamukong, Ivan; Lapko, Hanna; Cerny, Ronald; Avramova, Zoya V.

In: Nucleic acids research, Vol. 39, No. 11, 01.06.2011, p. 4709-4718.

Research output: Contribution to journalArticle

@article{4a8136b19fe44898a74cb349daf9bb4f,
title = "A cytoplasm-specific activity encoded by the Trithorax-like ATX1 gene",
abstract = "Eukaryotes produce multiple products from a single gene locus by alternative splicing, translation or promoter usage as mechanisms expanding the complexity of their proteome. Trithorax proteins, including the Arabidopsis Trithorax-like protein ATX1, are histone modifiers regulating gene activity. Here, we report that a novel member of the Trithorax family has a role unrelated to chromatin. It is encoded from an internal promoter in the ATX1 locus as an isoform containing only the SET domain (soloSET). It is located exclusively in the cytoplasm and its substrate is the elongation factor 1A (EF1A). Loss of SET, but not of the histone modifying ATX1-SET activity, affects cytoskeletal actin bundling illustrating that the two isoforms have distinct functions in Arabidopsis cells.",
author = "Ivan Ndamukong and Hanna Lapko and Ronald Cerny and Avramova, {Zoya V}",
year = "2011",
month = "6",
day = "1",
doi = "10.1093/nar/gkq1300",
language = "English (US)",
volume = "39",
pages = "4709--4718",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "11",

}

TY - JOUR

T1 - A cytoplasm-specific activity encoded by the Trithorax-like ATX1 gene

AU - Ndamukong, Ivan

AU - Lapko, Hanna

AU - Cerny, Ronald

AU - Avramova, Zoya V

PY - 2011/6/1

Y1 - 2011/6/1

N2 - Eukaryotes produce multiple products from a single gene locus by alternative splicing, translation or promoter usage as mechanisms expanding the complexity of their proteome. Trithorax proteins, including the Arabidopsis Trithorax-like protein ATX1, are histone modifiers regulating gene activity. Here, we report that a novel member of the Trithorax family has a role unrelated to chromatin. It is encoded from an internal promoter in the ATX1 locus as an isoform containing only the SET domain (soloSET). It is located exclusively in the cytoplasm and its substrate is the elongation factor 1A (EF1A). Loss of SET, but not of the histone modifying ATX1-SET activity, affects cytoskeletal actin bundling illustrating that the two isoforms have distinct functions in Arabidopsis cells.

AB - Eukaryotes produce multiple products from a single gene locus by alternative splicing, translation or promoter usage as mechanisms expanding the complexity of their proteome. Trithorax proteins, including the Arabidopsis Trithorax-like protein ATX1, are histone modifiers regulating gene activity. Here, we report that a novel member of the Trithorax family has a role unrelated to chromatin. It is encoded from an internal promoter in the ATX1 locus as an isoform containing only the SET domain (soloSET). It is located exclusively in the cytoplasm and its substrate is the elongation factor 1A (EF1A). Loss of SET, but not of the histone modifying ATX1-SET activity, affects cytoskeletal actin bundling illustrating that the two isoforms have distinct functions in Arabidopsis cells.

UR - http://www.scopus.com/inward/record.url?scp=79959394425&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959394425&partnerID=8YFLogxK

U2 - 10.1093/nar/gkq1300

DO - 10.1093/nar/gkq1300

M3 - Article

VL - 39

SP - 4709

EP - 4718

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 11

ER -