A cysteine endopeptidase isolated from castor bean endosperm microbodies processes the glyoxysomal malate dehydrogenase precursor protein

Christine Gietl, Bernhard Wimmer, Jiri Adamec, Frantisek Kalousek

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A plant cysteine endopeptidase with a molecular mass of 35 kD was purified from microbodies of germinating castor bean (Ricinus communis) endosperm by virtue of its capacity to specifically process the glyoxysomal malate dehydrogenase precursor protein to the mature subunit in vitro. Processing of the glyoxysomal malate dehydrogenase precursor occurs sequentially in three steps, the first intermediate resulting from cleavage after arginine-13 within the presequence and the second from cleavage after arginine-33. The endopeptidase is unable to remove the presequences of prethiolases from rape (Brassica napus) glyoxysomes and rat peroxisomes at the expected cleavage site. Protein sequence analysis of N-terminal and internal peptides revealed high identity to the mature papain-type cysteine endopeptidases from cotyledons of germinating mung bean (Vigna mungo) and French bean (Phaseolus vulgaris) seeds. These endopeptidases are synthesized with an extended pre-/prosequence at the N terminus and have been considered to be processed in the endoplasmic reticulum and targeted to protein-storing vacuoles.

Original languageEnglish (US)
Pages (from-to)863-871
Number of pages9
JournalPlant Physiology
Volume113
Issue number3
DOIs
StatePublished - Jan 1 1997

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Cysteine Endopeptidases
Castor Bean
Microbodies
microbodies
Endopeptidases
Malate Dehydrogenase
Endosperm
castor beans
Protein Precursors
cysteine proteinases
malate dehydrogenase
mung beans
endosperm
arginine
Arginine
Glyoxysomes
proteinases
glyoxysomes
Ricinus
Brassica napus

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

A cysteine endopeptidase isolated from castor bean endosperm microbodies processes the glyoxysomal malate dehydrogenase precursor protein. / Gietl, Christine; Wimmer, Bernhard; Adamec, Jiri; Kalousek, Frantisek.

In: Plant Physiology, Vol. 113, No. 3, 01.01.1997, p. 863-871.

Research output: Contribution to journalArticle

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