A cruciform structural transition provides a molecular switch for chromosome structure and dynamics

Luda S. Shlyakhtenko, Peggy Hsieh, Michael Grigoriev, Vladimir N. Potaman, Richard R. Sinden, Yuri L. Lyubchenko

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The interaction between specific sites along a DNA molecule is often crucial for the regulation of genetic processes. However, mechanisms regulating the interaction of specific sites are unknown. We have used atomic force microscopy to demonstrate that the structural transition between cruciform conformations can act as a molecular switch to facilitate or prevent communication between distant regions in DNA. Cruciform structures exist in vivo and they are critically involved in the initiation of replication and the regulation of gene expression in different organisms. Therefore, structural transitions of the cruciform may play a key role in these processes. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)1169-1173
Number of pages5
JournalJournal of Molecular Biology
Volume296
Issue number5
DOIs
StatePublished - Mar 10 2000

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Chromosome Structures
Genetic Phenomena
Atomic Force Microscopy
DNA
Gene Expression Regulation

Keywords

  • Atomic force microscopy
  • Cruciform conformation
  • DNA supercoiling
  • Holliday junctions
  • Site-specific recombination

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

A cruciform structural transition provides a molecular switch for chromosome structure and dynamics. / Shlyakhtenko, Luda S.; Hsieh, Peggy; Grigoriev, Michael; Potaman, Vladimir N.; Sinden, Richard R.; Lyubchenko, Yuri L.

In: Journal of Molecular Biology, Vol. 296, No. 5, 10.03.2000, p. 1169-1173.

Research output: Contribution to journalArticle

Shlyakhtenko, Luda S. ; Hsieh, Peggy ; Grigoriev, Michael ; Potaman, Vladimir N. ; Sinden, Richard R. ; Lyubchenko, Yuri L. / A cruciform structural transition provides a molecular switch for chromosome structure and dynamics. In: Journal of Molecular Biology. 2000 ; Vol. 296, No. 5. pp. 1169-1173.
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