A comprehensive expression analysis of mucins in appendiceal carcinoma in a multicenter study: MUC3 is a novel prognostic factor

Hiroaki Shibahara, Michiyo Higashi, Seiya Yokoyama, Karine Rousseau, Iwao Kitazono, Masahiko Osako, Hiroshi Shirahama, Yukie Tashiro, Yasuhiro Kurumiya, Michihiko Narita, Shingo Kuze, Hiroshi Hasagawa, Takehito Kato, Hitoshi Kubota, Hideaki Suzuki, Toshiyuki Arai, Yu Sakai, Norihiro Yuasa, Masahiko Fujino, Shinji KondoYoshichika Okamoto, Tatsuyoshi Yamamoto, Takashi Hiromatsu, Eiji Sasaki, Kazuhisa Shirai, Satoru Kawai, Koutarou Hattori, Hideki Tsuji, Osamu Okochi, Masaki Sakamoto, Akinobu Kondo, Naomi Konishi, Surinder Kumar Batra, Suguru Yonezawa

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Mucins are implicated in survival in various cancers, but there have been no report addressed on survival in appendiceal carcinoma, an uncommon disease with different clinical and pathological features from those of other colon cancers. We aimed to investigate the clinical implications of expression of mucins in appendiceal carcinoma. Methods: Expression profiles of MUC1, MUC2, MUC3, MUC4, MUC5AC, MUC6, MUC16 and MUC17 in cancer tissue were examined by immunohistochemistry in 108 cases of surgically resected appendiceal carcinoma. Results: The following relationships of mucins with clinicopathologic factors were identified: MUC1 with positive lymphatic invasion (p=0.036); MUC2 with histological type (mucinous carcinoma, p<0.001), superficial invasion depth (p=0.007), negative venous invasion (p=0.003), and curative resection (p=0.019); MUC3 with non-curative resection (p=0.017); MUC5AC with histological type (mucinous carcinoma, p=0.002), negative lymphatic invasion (p=0.021), and negative venous invasion (p=0.022); and MUC16 with positive lymph node metastasis (p=0.035), positive venous invasion (p<0.05), and non-curative resection (p=0.035). A poor prognosis was related to positive lymph node metastasis (p=0.04), positive lymphatic invasion (p=0.02), positive venous invasion (p<0.001), non-curative resection (p<0.001), and positive expression of MUC3 (p=0.004). In multivariate analysis, positive venous invasion (HR: 6.93, 95% CI: 1.93-24.96, p=0.003), non-curative resection (HR: 10.19, 95% CI: 3.05-34.07, p<0.001) and positive MUC3 expression (HR: 3.37, 95% CI: 1.13-10.03, p=0.03) were identified as significant independent prognostic factors in patients with appendiceal carcinoma. Conclusions: Expression of MUC3 in appendiceal carcinoma is an independent factor for poor prognosis and a useful predictor of outcome in patients with appendiceal carcinoma after surgery.

Original languageEnglish (US)
Article numbere115613
JournalPloS one
Volume9
Issue number12
DOIs
StatePublished - Dec 31 2014

Fingerprint

mucins
Mucins
Multicenter Studies
carcinoma
Carcinoma
resection
Mucinous Adenocarcinoma
Surgery
Lymph Nodes
metastasis
Tissue
Neoplasm Metastasis
prognosis
lymph nodes
Colonic Neoplasms
neoplasms
Neoplasms
Multivariate Analysis
Immunohistochemistry
colorectal neoplasms

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Shibahara, H., Higashi, M., Yokoyama, S., Rousseau, K., Kitazono, I., Osako, M., ... Yonezawa, S. (2014). A comprehensive expression analysis of mucins in appendiceal carcinoma in a multicenter study: MUC3 is a novel prognostic factor. PloS one, 9(12), [e115613]. https://doi.org/10.1371/journal.pone.0115613

A comprehensive expression analysis of mucins in appendiceal carcinoma in a multicenter study : MUC3 is a novel prognostic factor. / Shibahara, Hiroaki; Higashi, Michiyo; Yokoyama, Seiya; Rousseau, Karine; Kitazono, Iwao; Osako, Masahiko; Shirahama, Hiroshi; Tashiro, Yukie; Kurumiya, Yasuhiro; Narita, Michihiko; Kuze, Shingo; Hasagawa, Hiroshi; Kato, Takehito; Kubota, Hitoshi; Suzuki, Hideaki; Arai, Toshiyuki; Sakai, Yu; Yuasa, Norihiro; Fujino, Masahiko; Kondo, Shinji; Okamoto, Yoshichika; Yamamoto, Tatsuyoshi; Hiromatsu, Takashi; Sasaki, Eiji; Shirai, Kazuhisa; Kawai, Satoru; Hattori, Koutarou; Tsuji, Hideki; Okochi, Osamu; Sakamoto, Masaki; Kondo, Akinobu; Konishi, Naomi; Batra, Surinder Kumar; Yonezawa, Suguru.

In: PloS one, Vol. 9, No. 12, e115613, 31.12.2014.

Research output: Contribution to journalArticle

Shibahara, H, Higashi, M, Yokoyama, S, Rousseau, K, Kitazono, I, Osako, M, Shirahama, H, Tashiro, Y, Kurumiya, Y, Narita, M, Kuze, S, Hasagawa, H, Kato, T, Kubota, H, Suzuki, H, Arai, T, Sakai, Y, Yuasa, N, Fujino, M, Kondo, S, Okamoto, Y, Yamamoto, T, Hiromatsu, T, Sasaki, E, Shirai, K, Kawai, S, Hattori, K, Tsuji, H, Okochi, O, Sakamoto, M, Kondo, A, Konishi, N, Batra, SK & Yonezawa, S 2014, 'A comprehensive expression analysis of mucins in appendiceal carcinoma in a multicenter study: MUC3 is a novel prognostic factor', PloS one, vol. 9, no. 12, e115613. https://doi.org/10.1371/journal.pone.0115613
Shibahara, Hiroaki ; Higashi, Michiyo ; Yokoyama, Seiya ; Rousseau, Karine ; Kitazono, Iwao ; Osako, Masahiko ; Shirahama, Hiroshi ; Tashiro, Yukie ; Kurumiya, Yasuhiro ; Narita, Michihiko ; Kuze, Shingo ; Hasagawa, Hiroshi ; Kato, Takehito ; Kubota, Hitoshi ; Suzuki, Hideaki ; Arai, Toshiyuki ; Sakai, Yu ; Yuasa, Norihiro ; Fujino, Masahiko ; Kondo, Shinji ; Okamoto, Yoshichika ; Yamamoto, Tatsuyoshi ; Hiromatsu, Takashi ; Sasaki, Eiji ; Shirai, Kazuhisa ; Kawai, Satoru ; Hattori, Koutarou ; Tsuji, Hideki ; Okochi, Osamu ; Sakamoto, Masaki ; Kondo, Akinobu ; Konishi, Naomi ; Batra, Surinder Kumar ; Yonezawa, Suguru. / A comprehensive expression analysis of mucins in appendiceal carcinoma in a multicenter study : MUC3 is a novel prognostic factor. In: PloS one. 2014 ; Vol. 9, No. 12.
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title = "A comprehensive expression analysis of mucins in appendiceal carcinoma in a multicenter study: MUC3 is a novel prognostic factor",
abstract = "Background: Mucins are implicated in survival in various cancers, but there have been no report addressed on survival in appendiceal carcinoma, an uncommon disease with different clinical and pathological features from those of other colon cancers. We aimed to investigate the clinical implications of expression of mucins in appendiceal carcinoma. Methods: Expression profiles of MUC1, MUC2, MUC3, MUC4, MUC5AC, MUC6, MUC16 and MUC17 in cancer tissue were examined by immunohistochemistry in 108 cases of surgically resected appendiceal carcinoma. Results: The following relationships of mucins with clinicopathologic factors were identified: MUC1 with positive lymphatic invasion (p=0.036); MUC2 with histological type (mucinous carcinoma, p<0.001), superficial invasion depth (p=0.007), negative venous invasion (p=0.003), and curative resection (p=0.019); MUC3 with non-curative resection (p=0.017); MUC5AC with histological type (mucinous carcinoma, p=0.002), negative lymphatic invasion (p=0.021), and negative venous invasion (p=0.022); and MUC16 with positive lymph node metastasis (p=0.035), positive venous invasion (p<0.05), and non-curative resection (p=0.035). A poor prognosis was related to positive lymph node metastasis (p=0.04), positive lymphatic invasion (p=0.02), positive venous invasion (p<0.001), non-curative resection (p<0.001), and positive expression of MUC3 (p=0.004). In multivariate analysis, positive venous invasion (HR: 6.93, 95{\%} CI: 1.93-24.96, p=0.003), non-curative resection (HR: 10.19, 95{\%} CI: 3.05-34.07, p<0.001) and positive MUC3 expression (HR: 3.37, 95{\%} CI: 1.13-10.03, p=0.03) were identified as significant independent prognostic factors in patients with appendiceal carcinoma. Conclusions: Expression of MUC3 in appendiceal carcinoma is an independent factor for poor prognosis and a useful predictor of outcome in patients with appendiceal carcinoma after surgery.",
author = "Hiroaki Shibahara and Michiyo Higashi and Seiya Yokoyama and Karine Rousseau and Iwao Kitazono and Masahiko Osako and Hiroshi Shirahama and Yukie Tashiro and Yasuhiro Kurumiya and Michihiko Narita and Shingo Kuze and Hiroshi Hasagawa and Takehito Kato and Hitoshi Kubota and Hideaki Suzuki and Toshiyuki Arai and Yu Sakai and Norihiro Yuasa and Masahiko Fujino and Shinji Kondo and Yoshichika Okamoto and Tatsuyoshi Yamamoto and Takashi Hiromatsu and Eiji Sasaki and Kazuhisa Shirai and Satoru Kawai and Koutarou Hattori and Hideki Tsuji and Osamu Okochi and Masaki Sakamoto and Akinobu Kondo and Naomi Konishi and Batra, {Surinder Kumar} and Suguru Yonezawa",
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TY - JOUR

T1 - A comprehensive expression analysis of mucins in appendiceal carcinoma in a multicenter study

T2 - MUC3 is a novel prognostic factor

AU - Shibahara, Hiroaki

AU - Higashi, Michiyo

AU - Yokoyama, Seiya

AU - Rousseau, Karine

AU - Kitazono, Iwao

AU - Osako, Masahiko

AU - Shirahama, Hiroshi

AU - Tashiro, Yukie

AU - Kurumiya, Yasuhiro

AU - Narita, Michihiko

AU - Kuze, Shingo

AU - Hasagawa, Hiroshi

AU - Kato, Takehito

AU - Kubota, Hitoshi

AU - Suzuki, Hideaki

AU - Arai, Toshiyuki

AU - Sakai, Yu

AU - Yuasa, Norihiro

AU - Fujino, Masahiko

AU - Kondo, Shinji

AU - Okamoto, Yoshichika

AU - Yamamoto, Tatsuyoshi

AU - Hiromatsu, Takashi

AU - Sasaki, Eiji

AU - Shirai, Kazuhisa

AU - Kawai, Satoru

AU - Hattori, Koutarou

AU - Tsuji, Hideki

AU - Okochi, Osamu

AU - Sakamoto, Masaki

AU - Kondo, Akinobu

AU - Konishi, Naomi

AU - Batra, Surinder Kumar

AU - Yonezawa, Suguru

PY - 2014/12/31

Y1 - 2014/12/31

N2 - Background: Mucins are implicated in survival in various cancers, but there have been no report addressed on survival in appendiceal carcinoma, an uncommon disease with different clinical and pathological features from those of other colon cancers. We aimed to investigate the clinical implications of expression of mucins in appendiceal carcinoma. Methods: Expression profiles of MUC1, MUC2, MUC3, MUC4, MUC5AC, MUC6, MUC16 and MUC17 in cancer tissue were examined by immunohistochemistry in 108 cases of surgically resected appendiceal carcinoma. Results: The following relationships of mucins with clinicopathologic factors were identified: MUC1 with positive lymphatic invasion (p=0.036); MUC2 with histological type (mucinous carcinoma, p<0.001), superficial invasion depth (p=0.007), negative venous invasion (p=0.003), and curative resection (p=0.019); MUC3 with non-curative resection (p=0.017); MUC5AC with histological type (mucinous carcinoma, p=0.002), negative lymphatic invasion (p=0.021), and negative venous invasion (p=0.022); and MUC16 with positive lymph node metastasis (p=0.035), positive venous invasion (p<0.05), and non-curative resection (p=0.035). A poor prognosis was related to positive lymph node metastasis (p=0.04), positive lymphatic invasion (p=0.02), positive venous invasion (p<0.001), non-curative resection (p<0.001), and positive expression of MUC3 (p=0.004). In multivariate analysis, positive venous invasion (HR: 6.93, 95% CI: 1.93-24.96, p=0.003), non-curative resection (HR: 10.19, 95% CI: 3.05-34.07, p<0.001) and positive MUC3 expression (HR: 3.37, 95% CI: 1.13-10.03, p=0.03) were identified as significant independent prognostic factors in patients with appendiceal carcinoma. Conclusions: Expression of MUC3 in appendiceal carcinoma is an independent factor for poor prognosis and a useful predictor of outcome in patients with appendiceal carcinoma after surgery.

AB - Background: Mucins are implicated in survival in various cancers, but there have been no report addressed on survival in appendiceal carcinoma, an uncommon disease with different clinical and pathological features from those of other colon cancers. We aimed to investigate the clinical implications of expression of mucins in appendiceal carcinoma. Methods: Expression profiles of MUC1, MUC2, MUC3, MUC4, MUC5AC, MUC6, MUC16 and MUC17 in cancer tissue were examined by immunohistochemistry in 108 cases of surgically resected appendiceal carcinoma. Results: The following relationships of mucins with clinicopathologic factors were identified: MUC1 with positive lymphatic invasion (p=0.036); MUC2 with histological type (mucinous carcinoma, p<0.001), superficial invasion depth (p=0.007), negative venous invasion (p=0.003), and curative resection (p=0.019); MUC3 with non-curative resection (p=0.017); MUC5AC with histological type (mucinous carcinoma, p=0.002), negative lymphatic invasion (p=0.021), and negative venous invasion (p=0.022); and MUC16 with positive lymph node metastasis (p=0.035), positive venous invasion (p<0.05), and non-curative resection (p=0.035). A poor prognosis was related to positive lymph node metastasis (p=0.04), positive lymphatic invasion (p=0.02), positive venous invasion (p<0.001), non-curative resection (p<0.001), and positive expression of MUC3 (p=0.004). In multivariate analysis, positive venous invasion (HR: 6.93, 95% CI: 1.93-24.96, p=0.003), non-curative resection (HR: 10.19, 95% CI: 3.05-34.07, p<0.001) and positive MUC3 expression (HR: 3.37, 95% CI: 1.13-10.03, p=0.03) were identified as significant independent prognostic factors in patients with appendiceal carcinoma. Conclusions: Expression of MUC3 in appendiceal carcinoma is an independent factor for poor prognosis and a useful predictor of outcome in patients with appendiceal carcinoma after surgery.

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