A comparison of two techniques for nondestructive measurement of chlorophyll content in grapevine leaves

Mark R. Steele, Anatoly A. Gitelson, Donald C. Rundquist

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Traditional methods for chlorophyll (Chl) measurement include wet chemical extractions and handheld Chl meters. The recent availability of small and affordable radiometers has provided means to estimate Chl from reflectance measurements. This paper compares the performance of a handheld SPAD Chl meter and a recently developed Red Edge Chlorophyll Index (CIred edge) for Chl estimation in grapevine (Vitis spp.) leaves. Leaves were sampled and Chl was quantified using these two methods. Both techniques were compared against grapevine-leaf Chl as analyzed using standard laboratory procedures. Both SPAD and CIred edge were equally accurate in measuring Chl < 300 mg/m2. However, at higher Chl, SPAD sensitivity declined and the CIred edge accuracy was much higher than that of SPAD. The Chl index was found to be capable of accurately estimating pigment contents across a greater range than the SPAD, thus it can be used for quantitative assessment of early stages of plant stress.

Original languageEnglish (US)
Pages (from-to)779-782
Number of pages4
JournalAgronomy Journal
Volume100
Issue number3
DOIs
StatePublished - May 1 2008

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chlorophyll
leaves
methodology
meters (equipment)
radiometers
Vitis
plant stress
reflectance
pigments

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

A comparison of two techniques for nondestructive measurement of chlorophyll content in grapevine leaves. / Steele, Mark R.; Gitelson, Anatoly A.; Rundquist, Donald C.

In: Agronomy Journal, Vol. 100, No. 3, 01.05.2008, p. 779-782.

Research output: Contribution to journalArticle

Steele, Mark R. ; Gitelson, Anatoly A. ; Rundquist, Donald C. / A comparison of two techniques for nondestructive measurement of chlorophyll content in grapevine leaves. In: Agronomy Journal. 2008 ; Vol. 100, No. 3. pp. 779-782.
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