A comparison of the genomes of polioviruses by cDNA:RNA hybridization

Steven M Tracy, R. A. Smith

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

RNAs from 11 strains of the three serotypes of poliovirus were isolated from infected HeLa cells and hybridized with representative, radioactive complementary DNA (cDNA) from either poliovirus type 1 or type 2. Hybridization and thermal denaturation were analysed by hydroxyapatite chromatography. cDNA to either poliovirus type 1 or 2 is competent to detect about 70 to 80% of the sequences in RNAs from heterotypic strains and more than 92% of the RNA sequences in homotypic strains. The three polio serotype genomes appear to have diverged from each other by about 20%. Both poliovirus type 1 and type 2 representative cDNA can be used for the detection of poliovirus genomic sequences in RNA isolated from tissue.

Original languageEnglish (US)
Pages (from-to)193-199
Number of pages7
JournalJournal of General Virology
Volume55
Issue number1
DOIs
StatePublished - Jan 1 1981

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Complementary RNA
Poliovirus
Complementary DNA
Genome
Poliomyelitis
Durapatite
HeLa Cells
Chromatography
Hot Temperature
RNA

ASJC Scopus subject areas

  • Virology

Cite this

A comparison of the genomes of polioviruses by cDNA:RNA hybridization. / Tracy, Steven M; Smith, R. A.

In: Journal of General Virology, Vol. 55, No. 1, 01.01.1981, p. 193-199.

Research output: Contribution to journalArticle

Tracy, Steven M ; Smith, R. A. / A comparison of the genomes of polioviruses by cDNA:RNA hybridization. In: Journal of General Virology. 1981 ; Vol. 55, No. 1. pp. 193-199.
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