A comparison of the effects of betaine and S-adenosylmethionine on ethanol-induced changes in methionine metabolism and steatosis in rat hepatocytes

Kusum Kharbanda, David D. Rogers, Mark E Mailliard, Gerri L. Siford, Anthony J. Barak, Harriet C. Beckenhauer, Michael Floyd Sorrell, Dean J. Tuma

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Previous studies showed that chronic ethanol administration alters methionine metabolism in the liver, resulting in increased intracellular S-adenosylhomocysteine (SAH) levels and increased homocysteine release into the plasma. We showed further that these changes appear to be reversed by betaine administration. This study compared the effects of betaine and S-adenosylmethionine (SAM), another methylating agent, on ethanol-induced changes of methionine metabolism and hepatic steaiosis. Wistar rats were fed ethanol or control Lieber-Decarli liquid diet for 4 wk and metabolites of the methionine cycle were measured in isolated hepatocytes. Hepatocytes from ethanol-fed rats had a 50% lower intracellular SAM:SAH ratio and almost 2-fold greater homocysteine release into the media compared with controls. Supplementation of betaine or SAM in the incubation media increased this ratio in hepatocytes from both control and ethanol-fed rats and attenuated the ethanol-induced increased hepatocellular triglyceride levels by ∼20%. On the other hand, only betaine prevented the increase in generation of homocysteine in the incubation media under basal and methionine-loaded conditions. SAM can correct only the ratio and the methylation defects and may in fact be detrimental after prolonged use because of its propensity to increase homocysteine release. Both SAM and betaine are effective in increasing the SAM:SAH ratio in hepatocytes and in attenuating hepatic steatosis; however, only betaine can effectively methylate homocysteine and prevent increased homocysteine release by the liver.

Original languageEnglish (US)
Pages (from-to)519-524
Number of pages6
JournalJournal of Nutrition
Volume135
Issue number3
StatePublished - Mar 1 2005

Fingerprint

S-Adenosylmethionine
Betaine
S-adenosylmethionine
homocysteine
betaine
Homocysteine
Methionine
hepatocytes
methionine
Hepatocytes
Ethanol
ethanol
adenosylhomocysteine
S-Adenosylhomocysteine
metabolism
rats
Liver
liver
liquid diet
fatty liver

Keywords

  • Betaine
  • Ethanol
  • Hepatocytes
  • S-adenosylhomocysteine
  • S-adenosylmethionine

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

A comparison of the effects of betaine and S-adenosylmethionine on ethanol-induced changes in methionine metabolism and steatosis in rat hepatocytes. / Kharbanda, Kusum; Rogers, David D.; Mailliard, Mark E; Siford, Gerri L.; Barak, Anthony J.; Beckenhauer, Harriet C.; Sorrell, Michael Floyd; Tuma, Dean J.

In: Journal of Nutrition, Vol. 135, No. 3, 01.03.2005, p. 519-524.

Research output: Contribution to journalArticle

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