A comparison of experimental and theoretical melting maps for replicative form of φX174 DNA

Yu L. Lyubchenko, Yu A. Kalambet, V. I. Lyamichev, A. S. Borovik

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A previously elaborated technique for fixing a chosen partially melted state of DHA with glyoxal was used in a study of the melting process of the replicative form (RF III) of φX174DNA. Electron-microscopic maps corresponding to five points of the melting curve of RF III were obtained and compared with the theoretical melting maps obtained in (4) and (6). Ibis comparison clearly shows that only rigorous calculations (4) and not the ones proposed by Azbel (6,7) correctly predict the course of RP III melting.

Original languageEnglish (US)
Pages (from-to)1867-1876
Number of pages10
JournalNucleic acids research
Issue number6
StatePublished - Mar 25 1982


ASJC Scopus subject areas

  • Genetics

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