A comparison of complete mitochondrial genomes of silver carp hypophthalmichthys molitrix and bighead carp hypophthalmichthys nobilis

Implications for their taxonomic relationship and phylogeny

S. F. Li, J. W. Xu, Q. L. Yang, C. H. Wang, Q. Chen, D. C. Chapman, Guoqing Lu

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Based upon morphological characters, Silver carp Hypophthalmichthys molitrix and bighead carp Hypophthalmichthys nobilis (or Aristichthys nobilis) have been classified into either the same genus or two distinct genera. Consequently, the taxonomic relationship of the two species at the generic level remains equivocal. This issue is addressed by sequencing complete mitochondrial genomes of H. molitrix and H. nobilis, comparing their mitogenome organization, structure and sequence similarity, and conducting a comprehensive phylogenetic analysis of cyprinid species. As with other cyprinid fishes, the mitogenomes of the two species were structurally conserved, containing 37 genes including 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA (tRNAs) genes and a putative control region (D-loop). Sequence similarity between the two mitogenomes varied in different genes or regions, being highest in the tRNA genes (98·8%), lowest in the control region (89·4%) and intermediate in the protein-coding genes (94·2%). Analyses of the sequence comparison and phylogeny using concatenated protein sequences support the view that the two species belong to the genus Hypophthalmichthys. Further studies using nuclear markers and involving more closely related species, and the systematic combination of traditional biology and molecular biology are needed in order to confirm this conclusion.

Original languageEnglish (US)
Pages (from-to)1787-1803
Number of pages17
JournalJournal of Fish Biology
Volume74
Issue number8
DOIs
StatePublished - May 1 2009

Fingerprint

Hypophthalmichthys nobilis
Hypophthalmichthys molitrix
silver
phylogeny
genome
gene
RNA
genes
cyprinid
transfer RNA
protein
Hypophthalmichthys
gene transfer
mitochondrial genome
comparison
molecular biology
amino acid sequences
phylogenetics
proteins
ribosomal RNA

Keywords

  • Bighead carp
  • Generic taxonomy
  • Mitochondrial genome
  • Molecular phylogeny
  • Sequence comparison
  • Silver carp

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Cite this

A comparison of complete mitochondrial genomes of silver carp hypophthalmichthys molitrix and bighead carp hypophthalmichthys nobilis : Implications for their taxonomic relationship and phylogeny. / Li, S. F.; Xu, J. W.; Yang, Q. L.; Wang, C. H.; Chen, Q.; Chapman, D. C.; Lu, Guoqing.

In: Journal of Fish Biology, Vol. 74, No. 8, 01.05.2009, p. 1787-1803.

Research output: Contribution to journalArticle

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abstract = "Based upon morphological characters, Silver carp Hypophthalmichthys molitrix and bighead carp Hypophthalmichthys nobilis (or Aristichthys nobilis) have been classified into either the same genus or two distinct genera. Consequently, the taxonomic relationship of the two species at the generic level remains equivocal. This issue is addressed by sequencing complete mitochondrial genomes of H. molitrix and H. nobilis, comparing their mitogenome organization, structure and sequence similarity, and conducting a comprehensive phylogenetic analysis of cyprinid species. As with other cyprinid fishes, the mitogenomes of the two species were structurally conserved, containing 37 genes including 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA (tRNAs) genes and a putative control region (D-loop). Sequence similarity between the two mitogenomes varied in different genes or regions, being highest in the tRNA genes (98·8{\%}), lowest in the control region (89·4{\%}) and intermediate in the protein-coding genes (94·2{\%}). Analyses of the sequence comparison and phylogeny using concatenated protein sequences support the view that the two species belong to the genus Hypophthalmichthys. Further studies using nuclear markers and involving more closely related species, and the systematic combination of traditional biology and molecular biology are needed in order to confirm this conclusion.",
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