A comparative study of lysozyme and its secretion by tracheal epithelium

Michael W. Konstan, Pi-Wan Cheng, Thomas F. Boat

Research output: Contribution to journalArticle

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Abstract

Tracbeal explants from baboon, ferret, dog, mouse, hamster, rat, guinea pig, chicken, sheep, rabbit, and cat were maintained in vitro for 24 to 12 hr. Bathing media were analyzed for lysozyme content. Secretory rates were compared to the rate obtained for human trachea in our earlier work. These rates ranged from 807 μg lysozyme/g tissue/24 hr for human trachea to 11 μg lysozyme/g tissue/24 hr for chicken trachea. Secretory rates for baboon, ferret, dog, mouse, hamster, rat, and guinea pig tracheal lysozyme fell within this range in decreasing order. No detectable lysozyme was released from sheep, cat, or rabbit explants. Lysozyme secretion did not correlate with the presence or absence of submucosal glands. Lysozyme from laboratory animals in all cases differed with respect to electrophoretic mobility and immunochemical properties from human lysozyme. These observations provide physiological, physicochemical, and immunochemical information which will facilitate the use of lysozyme to assess secretion of macromolecules in in vitro and in vivo studies of airway epithelium.

Original languageEnglish (US)
Pages (from-to)175-181
Number of pages7
JournalExperimental Lung Research
Volume3
Issue number2
DOIs
StatePublished - Jan 1 1982

Fingerprint

Muramidase
Epithelium
Trachea
Secretory Rate
Ferrets
Papio
Cricetinae
Rats
Chickens
Sheep
Guinea Pigs
Cats
Dogs
Tissue
Rabbits
Electrophoretic mobility
Laboratory Animals
Macromolecules
Animals

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

A comparative study of lysozyme and its secretion by tracheal epithelium. / Konstan, Michael W.; Cheng, Pi-Wan; Boat, Thomas F.

In: Experimental Lung Research, Vol. 3, No. 2, 01.01.1982, p. 175-181.

Research output: Contribution to journalArticle

Konstan, Michael W. ; Cheng, Pi-Wan ; Boat, Thomas F. / A comparative study of lysozyme and its secretion by tracheal epithelium. In: Experimental Lung Research. 1982 ; Vol. 3, No. 2. pp. 175-181.
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