A comparative study of HIV-1 clade C env evolution in a Zambian infant with an infected rhesus macaque during disease progression

For Yue Tso, Federico G. Hoffmann, Damien C. Tully, Philippe Lemey, Robert A. Rasmussen, Hong Zhang, Ruth M. Ruprecht, Charles Wood

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objective: To evaluate whether HIV-1 clade C (HIV-C) envelope variations that arise during disease progression in rhesus macaque model reflect changes that occur naturally in human infection. Design: An infant macaque was infected with SHIV-1157i, an R5 tropic clade C SHIV, that expresses a primary HIV-C envelope derived from an infected human infant and monitored over a 5-year period. Genetic variation of the V1-V5 envelope region, which is the main target for humoral immune responses, derived from the infected macaque and infant was examined. Methods: The V1-V5 envelope region was cloned and sequenced from longitudinal peripheral blood mononuclear cell samples collected from the infected macaque and infant. Phylogenetic analysis [phylogenetic tree, diversity, divergence, ratio of nonsynonymous (dN) and synonymous substitution (dS) and dN distribution] was performed. Plasma RNA viral load, CD4 T-cell count, changes in the length of V1-V5 region, putative N-linked glycosylation site number and distribution were also measured. Results: Phylogenetic analysis revealed that changes in the macaque closely reflected those of the infant during disease progression. Similar distribution patterns of dN and hot spots were observed between the macaque and infant. Analysis of putative N-linked glycosylation sites revealed several common variations between the virus populations in the two host species. These variations correlate with decline of CD4 T-cell count in the macaque and might be linked with disease progression. Conclusion: SHIV-C infection of macaque is a relevant animal model for studying variation of primary HIV-C envelope during disease progression and could be used to analyze the selection pressures that are associated with those changes.

Original languageEnglish (US)
Pages (from-to)1817-1828
Number of pages12
JournalAIDS
Volume23
Issue number14
DOIs
StatePublished - Sep 1 2009

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Macaca
Macaca mulatta
Disease Progression
HIV-1
CD4 Lymphocyte Count
Glycosylation
T-Lymphocytes
Humoral Immunity
Infection
Viral Load
Blood Cells
Animal Models
RNA
Viruses
Pressure
Population

Keywords

  • Disease progression
  • Evolution
  • HIV-1
  • Rhesus macaque
  • Simian HIV

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Cite this

A comparative study of HIV-1 clade C env evolution in a Zambian infant with an infected rhesus macaque during disease progression. / Tso, For Yue; Hoffmann, Federico G.; Tully, Damien C.; Lemey, Philippe; Rasmussen, Robert A.; Zhang, Hong; Ruprecht, Ruth M.; Wood, Charles.

In: AIDS, Vol. 23, No. 14, 01.09.2009, p. 1817-1828.

Research output: Contribution to journalArticle

Tso, FY, Hoffmann, FG, Tully, DC, Lemey, P, Rasmussen, RA, Zhang, H, Ruprecht, RM & Wood, C 2009, 'A comparative study of HIV-1 clade C env evolution in a Zambian infant with an infected rhesus macaque during disease progression', AIDS, vol. 23, no. 14, pp. 1817-1828. https://doi.org/10.1097/QAD.0b013e32832f3da6
Tso, For Yue ; Hoffmann, Federico G. ; Tully, Damien C. ; Lemey, Philippe ; Rasmussen, Robert A. ; Zhang, Hong ; Ruprecht, Ruth M. ; Wood, Charles. / A comparative study of HIV-1 clade C env evolution in a Zambian infant with an infected rhesus macaque during disease progression. In: AIDS. 2009 ; Vol. 23, No. 14. pp. 1817-1828.
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abstract = "Objective: To evaluate whether HIV-1 clade C (HIV-C) envelope variations that arise during disease progression in rhesus macaque model reflect changes that occur naturally in human infection. Design: An infant macaque was infected with SHIV-1157i, an R5 tropic clade C SHIV, that expresses a primary HIV-C envelope derived from an infected human infant and monitored over a 5-year period. Genetic variation of the V1-V5 envelope region, which is the main target for humoral immune responses, derived from the infected macaque and infant was examined. Methods: The V1-V5 envelope region was cloned and sequenced from longitudinal peripheral blood mononuclear cell samples collected from the infected macaque and infant. Phylogenetic analysis [phylogenetic tree, diversity, divergence, ratio of nonsynonymous (dN) and synonymous substitution (dS) and dN distribution] was performed. Plasma RNA viral load, CD4 T-cell count, changes in the length of V1-V5 region, putative N-linked glycosylation site number and distribution were also measured. Results: Phylogenetic analysis revealed that changes in the macaque closely reflected those of the infant during disease progression. Similar distribution patterns of dN and hot spots were observed between the macaque and infant. Analysis of putative N-linked glycosylation sites revealed several common variations between the virus populations in the two host species. These variations correlate with decline of CD4 T-cell count in the macaque and might be linked with disease progression. Conclusion: SHIV-C infection of macaque is a relevant animal model for studying variation of primary HIV-C envelope during disease progression and could be used to analyze the selection pressures that are associated with those changes.",
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AU - Tully, Damien C.

AU - Lemey, Philippe

AU - Rasmussen, Robert A.

AU - Zhang, Hong

AU - Ruprecht, Ruth M.

AU - Wood, Charles

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N2 - Objective: To evaluate whether HIV-1 clade C (HIV-C) envelope variations that arise during disease progression in rhesus macaque model reflect changes that occur naturally in human infection. Design: An infant macaque was infected with SHIV-1157i, an R5 tropic clade C SHIV, that expresses a primary HIV-C envelope derived from an infected human infant and monitored over a 5-year period. Genetic variation of the V1-V5 envelope region, which is the main target for humoral immune responses, derived from the infected macaque and infant was examined. Methods: The V1-V5 envelope region was cloned and sequenced from longitudinal peripheral blood mononuclear cell samples collected from the infected macaque and infant. Phylogenetic analysis [phylogenetic tree, diversity, divergence, ratio of nonsynonymous (dN) and synonymous substitution (dS) and dN distribution] was performed. Plasma RNA viral load, CD4 T-cell count, changes in the length of V1-V5 region, putative N-linked glycosylation site number and distribution were also measured. Results: Phylogenetic analysis revealed that changes in the macaque closely reflected those of the infant during disease progression. Similar distribution patterns of dN and hot spots were observed between the macaque and infant. Analysis of putative N-linked glycosylation sites revealed several common variations between the virus populations in the two host species. These variations correlate with decline of CD4 T-cell count in the macaque and might be linked with disease progression. Conclusion: SHIV-C infection of macaque is a relevant animal model for studying variation of primary HIV-C envelope during disease progression and could be used to analyze the selection pressures that are associated with those changes.

AB - Objective: To evaluate whether HIV-1 clade C (HIV-C) envelope variations that arise during disease progression in rhesus macaque model reflect changes that occur naturally in human infection. Design: An infant macaque was infected with SHIV-1157i, an R5 tropic clade C SHIV, that expresses a primary HIV-C envelope derived from an infected human infant and monitored over a 5-year period. Genetic variation of the V1-V5 envelope region, which is the main target for humoral immune responses, derived from the infected macaque and infant was examined. Methods: The V1-V5 envelope region was cloned and sequenced from longitudinal peripheral blood mononuclear cell samples collected from the infected macaque and infant. Phylogenetic analysis [phylogenetic tree, diversity, divergence, ratio of nonsynonymous (dN) and synonymous substitution (dS) and dN distribution] was performed. Plasma RNA viral load, CD4 T-cell count, changes in the length of V1-V5 region, putative N-linked glycosylation site number and distribution were also measured. Results: Phylogenetic analysis revealed that changes in the macaque closely reflected those of the infant during disease progression. Similar distribution patterns of dN and hot spots were observed between the macaque and infant. Analysis of putative N-linked glycosylation sites revealed several common variations between the virus populations in the two host species. These variations correlate with decline of CD4 T-cell count in the macaque and might be linked with disease progression. Conclusion: SHIV-C infection of macaque is a relevant animal model for studying variation of primary HIV-C envelope during disease progression and could be used to analyze the selection pressures that are associated with those changes.

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KW - Simian HIV

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