A comparative analysis of the mitogenic response of intestinal intraepithelial lymphocytes in various age groups of turkeys

P. K. Agrawal, D. L. Reynolds

Research output: Contribution to journalReview article

4 Citations (Scopus)

Abstract

Mitogenic responsiveness of intestinal intraepithelial lymphocytes (i-IEL) to concanavalin A (Con A), phytohemagglutinin P (PHA-P), and lipopolysaccharide (LPS) from Salmonella typhimurium were evaluated in various age groups of turkeys by a colorimetric blastogenic microassay. Comparisons were made between mitogenic responses of turkey i-IEL and peripheral blood lymphocytes (PBL). The results from this study demonstrated that i-IEL and PBL of turkeys responded to T-cell mitogens, Con A and PHA-P, in every age group examined. The LPS induced a significant mitogenic response in PBL but not in i-IEL of turkeys. The mitogenic responses of turkey i-IEL and PBL to the three mitogens examined were similar to mitogenic responses observed in an earlier study performed by using chicken i-IEL and PBL. The results indicated a difference in mitogenic response between different age groups. An increase was found in mitogenic response of i-IEL to both T-cell mitogens from 3 days of age to 1 wk of age, whereas mitogenic response of PBL to all three mitogens declined significantly from 1 day of age to 3 days of age. The highest mitogenic response of i-IEL to T-cell mitogens was observed at 1 wk of age. The highest mitogenic response of PBL to both T-cell mitogens was observed at 1 day of age and the highest PBL response to LPS was observed at 16 wk of age. The mitogenic response induced by PHA-P provided less variability between age groups than the mitogenic response induced by Con A.

Original languageEnglish (US)
Pages (from-to)376-383
Number of pages8
JournalAvian diseases
Volume43
Issue number3
DOIs
StatePublished - Jan 1 1999

Fingerprint

lymphocytes
Age Groups
Lymphocytes
Mitogens
blood
phytohemagglutinin
T-lymphocytes
lipopolysaccharides
T-Lymphocytes
Lipopolysaccharides
concanavalin A
Concanavalin A
Salmonella typhimurium
Salmonella Typhimurium
Chickens
chickens

Keywords

  • Cell-mediated immune response
  • Gut-associated lymphoid tissue
  • Intestinal intraepithelial lymphocytes
  • Mitogenic response

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Immunology and Microbiology(all)

Cite this

A comparative analysis of the mitogenic response of intestinal intraepithelial lymphocytes in various age groups of turkeys. / Agrawal, P. K.; Reynolds, D. L.

In: Avian diseases, Vol. 43, No. 3, 01.01.1999, p. 376-383.

Research output: Contribution to journalReview article

@article{5d09005cf2734a1dabc4fd6253c53721,
title = "A comparative analysis of the mitogenic response of intestinal intraepithelial lymphocytes in various age groups of turkeys",
abstract = "Mitogenic responsiveness of intestinal intraepithelial lymphocytes (i-IEL) to concanavalin A (Con A), phytohemagglutinin P (PHA-P), and lipopolysaccharide (LPS) from Salmonella typhimurium were evaluated in various age groups of turkeys by a colorimetric blastogenic microassay. Comparisons were made between mitogenic responses of turkey i-IEL and peripheral blood lymphocytes (PBL). The results from this study demonstrated that i-IEL and PBL of turkeys responded to T-cell mitogens, Con A and PHA-P, in every age group examined. The LPS induced a significant mitogenic response in PBL but not in i-IEL of turkeys. The mitogenic responses of turkey i-IEL and PBL to the three mitogens examined were similar to mitogenic responses observed in an earlier study performed by using chicken i-IEL and PBL. The results indicated a difference in mitogenic response between different age groups. An increase was found in mitogenic response of i-IEL to both T-cell mitogens from 3 days of age to 1 wk of age, whereas mitogenic response of PBL to all three mitogens declined significantly from 1 day of age to 3 days of age. The highest mitogenic response of i-IEL to T-cell mitogens was observed at 1 wk of age. The highest mitogenic response of PBL to both T-cell mitogens was observed at 1 day of age and the highest PBL response to LPS was observed at 16 wk of age. The mitogenic response induced by PHA-P provided less variability between age groups than the mitogenic response induced by Con A.",
keywords = "Cell-mediated immune response, Gut-associated lymphoid tissue, Intestinal intraepithelial lymphocytes, Mitogenic response",
author = "Agrawal, {P. K.} and Reynolds, {D. L.}",
year = "1999",
month = "1",
day = "1",
doi = "10.2307/1592634",
language = "English (US)",
volume = "43",
pages = "376--383",
journal = "Avian Diseases",
issn = "0005-2086",
publisher = "American Association of Avian Pathologists",
number = "3",

}

TY - JOUR

T1 - A comparative analysis of the mitogenic response of intestinal intraepithelial lymphocytes in various age groups of turkeys

AU - Agrawal, P. K.

AU - Reynolds, D. L.

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Mitogenic responsiveness of intestinal intraepithelial lymphocytes (i-IEL) to concanavalin A (Con A), phytohemagglutinin P (PHA-P), and lipopolysaccharide (LPS) from Salmonella typhimurium were evaluated in various age groups of turkeys by a colorimetric blastogenic microassay. Comparisons were made between mitogenic responses of turkey i-IEL and peripheral blood lymphocytes (PBL). The results from this study demonstrated that i-IEL and PBL of turkeys responded to T-cell mitogens, Con A and PHA-P, in every age group examined. The LPS induced a significant mitogenic response in PBL but not in i-IEL of turkeys. The mitogenic responses of turkey i-IEL and PBL to the three mitogens examined were similar to mitogenic responses observed in an earlier study performed by using chicken i-IEL and PBL. The results indicated a difference in mitogenic response between different age groups. An increase was found in mitogenic response of i-IEL to both T-cell mitogens from 3 days of age to 1 wk of age, whereas mitogenic response of PBL to all three mitogens declined significantly from 1 day of age to 3 days of age. The highest mitogenic response of i-IEL to T-cell mitogens was observed at 1 wk of age. The highest mitogenic response of PBL to both T-cell mitogens was observed at 1 day of age and the highest PBL response to LPS was observed at 16 wk of age. The mitogenic response induced by PHA-P provided less variability between age groups than the mitogenic response induced by Con A.

AB - Mitogenic responsiveness of intestinal intraepithelial lymphocytes (i-IEL) to concanavalin A (Con A), phytohemagglutinin P (PHA-P), and lipopolysaccharide (LPS) from Salmonella typhimurium were evaluated in various age groups of turkeys by a colorimetric blastogenic microassay. Comparisons were made between mitogenic responses of turkey i-IEL and peripheral blood lymphocytes (PBL). The results from this study demonstrated that i-IEL and PBL of turkeys responded to T-cell mitogens, Con A and PHA-P, in every age group examined. The LPS induced a significant mitogenic response in PBL but not in i-IEL of turkeys. The mitogenic responses of turkey i-IEL and PBL to the three mitogens examined were similar to mitogenic responses observed in an earlier study performed by using chicken i-IEL and PBL. The results indicated a difference in mitogenic response between different age groups. An increase was found in mitogenic response of i-IEL to both T-cell mitogens from 3 days of age to 1 wk of age, whereas mitogenic response of PBL to all three mitogens declined significantly from 1 day of age to 3 days of age. The highest mitogenic response of i-IEL to T-cell mitogens was observed at 1 wk of age. The highest mitogenic response of PBL to both T-cell mitogens was observed at 1 day of age and the highest PBL response to LPS was observed at 16 wk of age. The mitogenic response induced by PHA-P provided less variability between age groups than the mitogenic response induced by Con A.

KW - Cell-mediated immune response

KW - Gut-associated lymphoid tissue

KW - Intestinal intraepithelial lymphocytes

KW - Mitogenic response

UR - http://www.scopus.com/inward/record.url?scp=0033167058&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033167058&partnerID=8YFLogxK

U2 - 10.2307/1592634

DO - 10.2307/1592634

M3 - Review article

C2 - 10494405

AN - SCOPUS:0033167058

VL - 43

SP - 376

EP - 383

JO - Avian Diseases

JF - Avian Diseases

SN - 0005-2086

IS - 3

ER -