A behavioral mechanistic investigation of the role of 5-HT1A receptors in the mediation of rat maternal behavior

Xiaonan Li, Xiaojing Ding, Ruiyong Wu, Leilei Chen, Jun Gao, Gang Hu, Ming Li

Research output: Contribution to journalArticle

Abstract

Previous work suggests that 5-HT1A receptors play a special role in rodent maternal aggression, but not in other aspects of maternal care (e.g. pup retrieval and nest building). The present study re-assessed the basic effects of 5-HT1A activation or blockade on various maternal responses in postpartum female rats. We also examined the possible behavioral mechanisms underlying the maternal effects of 5-HT1A. Sprague–Dawley mother rats were injected with a 5-HT1A agonist 8-OH-DPAT (0.1, 0.5 or 1.0 mg/kg, sc), a 5-HT1A antagonist WAY-101405 (0.1, 0.5 or 1.0 mg/kg, sc) or 0.9% saline solution on postpartum days 3, 5, and 7. Maternal behavior was tested 30 min before, 30 min, 120 min, and 240 min after the injection. Acute and repeated 8-OH-DPAT treatment significantly disrupted pup retrieval, pup licking, nursing, and nest building in a dose-dependent fashion, whereas WAY-101405 had no effect at the tested doses. The 5-HT1A receptor specificity of 8-OH-DPAT's action was confirmed as its maternal disruption effect was reversed by pretreatment of WAY-100635 (a highly selective 5-HT1A receptor antagonist). Subsequent pup preference test found that 8-OH-DPAT did not decrease the pup preference over a novel object, thus no inhibition on maternal motivation or maternal affect. The pup separation test and pup retrieval on an elevated plus maze test also failed to find any motivational and motor impairment effect with 8-OH-DPAT. However, 8-OH-DPAT at the maternal disruptive dose did disrupt the prepulse inhibition (a measure of attentional function) of acoustic startle response and enhanced the basal startle response. These findings suggest that stimulation of 5-HT1A receptors by 8-OH-DPAT impairs maternal care by partially interfering with the attentional processing or basal anxiety. More work is needed to further delineate the psychological and neuronal mechanisms underlying the maternal disruptive effect of 5-HT1A receptor activation.

LanguageEnglish (US)
Pages16-26
Number of pages11
JournalPharmacology Biochemistry and Behavior
Volume169
DOIs
StatePublished - Jun 1 2018

Fingerprint

Maternal Behavior
8-Hydroxy-2-(di-n-propylamino)tetralin
Receptor, Serotonin, 5-HT1A
Rats
Mothers
Startle Reflex
Serotonin 5-HT1 Receptor Antagonists
Postpartum Period
Chemical activation
Serotonin 5-HT1 Receptor Agonists
Nursing
hydroxide ion
Sodium Chloride
Aggression
Acoustics
Sprague Dawley Rats
Motivation
Rodentia
Anxiety
Psychology

Keywords

  • 8-OH-DPAT
  • Maternal behavior
  • PPI and startle
  • Pup preference
  • Pup separation
  • Serotonin
  • Serotonin 1A receptor
  • WAY-101405

ASJC Scopus subject areas

  • Biochemistry
  • Toxicology
  • Pharmacology
  • Clinical Biochemistry
  • Biological Psychiatry
  • Behavioral Neuroscience

Cite this

A behavioral mechanistic investigation of the role of 5-HT1A receptors in the mediation of rat maternal behavior. / Li, Xiaonan; Ding, Xiaojing; Wu, Ruiyong; Chen, Leilei; Gao, Jun; Hu, Gang; Li, Ming.

In: Pharmacology Biochemistry and Behavior, Vol. 169, 01.06.2018, p. 16-26.

Research output: Contribution to journalArticle

Li, Xiaonan ; Ding, Xiaojing ; Wu, Ruiyong ; Chen, Leilei ; Gao, Jun ; Hu, Gang ; Li, Ming. / A behavioral mechanistic investigation of the role of 5-HT1A receptors in the mediation of rat maternal behavior. In: Pharmacology Biochemistry and Behavior. 2018 ; Vol. 169. pp. 16-26.
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