A Bacterial Type III Effector Targets the Master Regulator of Salicylic Acid Signaling, NPR1, to Subvert Plant Immunity

Huan Chen, Jian Chen, Min Li, Ming Chang, Kaimei Xu, Zhenhua Shang, Yi Zhao, Ian Palmer, Yuqiang Zhang, Jon McGill, James R. Alfano, Marc T. Nishimura, Fengquan Liu, Zheng Qing Fu

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Most plant bacterial pathogens rely on type III effectors to cause diseases. Although it is well known that the plant hormone salicylic acid (SA) plays an essential role in defense, whether the master regulator of SA signaling, NPR1, is targeted by any plant pathogen effectors is unknown. SA facilitates the reduction of cytosolic NPR1 oligomers into monomers, which enter the nucleus and function as transcriptional coactivators of plant defense genes. We show that SA promotes the interaction between the Pseudomonas syringae type III effector AvrPtoB and NPR1. In the presence of SA, AvrPtoB mediates the degradation of NPR1 via the host 26S proteasome in a manner dependent on AvrPtoB's E3 ligase activity. Intriguingly, we found that NPR1 plays an important role in MAMP-triggered immunity (MTI), inducing the expression of MTI marker genes. Thus, this work uncovers a strategy in which AvrPtoB targets NPR1 and represses NPR1-dependent SA signaling, thereby subverting plant innate immunity. NPR1, as the key transcriptional regulator of salicylic acid signaling, plays a pivotal role in plant local and systemic immunity. Chen et al. find that Pseudomonas syringae type III effector AvrPtoB directly targets the activated form of NPR1 for ubiquitination-mediated degradation to inhibit target gene expression, thereby dampening plant immunity.

Original languageEnglish (US)
Pages (from-to)777-788.e7
JournalCell Host and Microbe
Volume22
Issue number6
DOIs
StatePublished - Dec 13 2017

Fingerprint

Plant Immunity
Salicylic Acid
Pseudomonas syringae
Immunity
Plant Genes
Plant Growth Regulators
Ubiquitin-Protein Ligases
Ubiquitination
Innate Immunity
Gene Expression

Keywords

  • 26S proteasome
  • E3 ligase
  • MAMP-triggered immunity
  • NPR1
  • Pseudomonas syringae
  • salicylic acid
  • systemic acquired resistance
  • type III effector

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

Cite this

A Bacterial Type III Effector Targets the Master Regulator of Salicylic Acid Signaling, NPR1, to Subvert Plant Immunity. / Chen, Huan; Chen, Jian; Li, Min; Chang, Ming; Xu, Kaimei; Shang, Zhenhua; Zhao, Yi; Palmer, Ian; Zhang, Yuqiang; McGill, Jon; Alfano, James R.; Nishimura, Marc T.; Liu, Fengquan; Fu, Zheng Qing.

In: Cell Host and Microbe, Vol. 22, No. 6, 13.12.2017, p. 777-788.e7.

Research output: Contribution to journalArticle

Chen, H, Chen, J, Li, M, Chang, M, Xu, K, Shang, Z, Zhao, Y, Palmer, I, Zhang, Y, McGill, J, Alfano, JR, Nishimura, MT, Liu, F & Fu, ZQ 2017, 'A Bacterial Type III Effector Targets the Master Regulator of Salicylic Acid Signaling, NPR1, to Subvert Plant Immunity', Cell Host and Microbe, vol. 22, no. 6, pp. 777-788.e7. https://doi.org/10.1016/j.chom.2017.10.019
Chen, Huan ; Chen, Jian ; Li, Min ; Chang, Ming ; Xu, Kaimei ; Shang, Zhenhua ; Zhao, Yi ; Palmer, Ian ; Zhang, Yuqiang ; McGill, Jon ; Alfano, James R. ; Nishimura, Marc T. ; Liu, Fengquan ; Fu, Zheng Qing. / A Bacterial Type III Effector Targets the Master Regulator of Salicylic Acid Signaling, NPR1, to Subvert Plant Immunity. In: Cell Host and Microbe. 2017 ; Vol. 22, No. 6. pp. 777-788.e7.
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AU - Li, Min

AU - Chang, Ming

AU - Xu, Kaimei

AU - Shang, Zhenhua

AU - Zhao, Yi

AU - Palmer, Ian

AU - Zhang, Yuqiang

AU - McGill, Jon

AU - Alfano, James R.

AU - Nishimura, Marc T.

AU - Liu, Fengquan

AU - Fu, Zheng Qing

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