A Bacterial Effector Co-opts Calmodulin to Target the Plant Microtubule Network

Ming Guo, Panya Kim, Guangyong Li, Christian G. Elowsky, James R. Alfano

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The bacterial pathogen Pseudomonas syringae depends on effector proteins secreted by its type III secretion system for the pathogenesis of plants. The majority of these effector proteins are known suppressors of immunity, but their plant targets remain elusive. Using Arabidopsis thaliana as a model host, we report that the HopE1 effector uses the host calcium sensor, calmodulin (CaM), as a co-factor to target the microtubule-associated protein 65 (MAP65), an important component of the microtubule network. HopE1 interacted with MAP65 in a CaM-dependent manner, resulting in MAP65-GFP dissociation from microtubules. Transgenic Arabidopsis expressing HopE1 had reduced secretion of the immunity protein PR-1 compared to wild-type plants. Additionally, Arabidopsis map65-1 mutants were immune deficient and were more susceptible to P. syringae. Our results suggest a virulence strategy in which a pathogen effector is activated by host calmodulin to target MAP65 and the microtubule network, thereby inhibiting cell wall-based extracellular immunity.

Original languageEnglish (US)
Pages (from-to)67-78
Number of pages12
JournalCell Host and Microbe
Volume19
Issue number1
DOIs
StatePublished - Jan 13 2016

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Microtubule-Associated Proteins
Calmodulin
Microtubules
Arabidopsis
Pseudomonas syringae
Immunity
Plant Immunity
Proteins
Cell Wall
Virulence
Calcium

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology and Microbiology(all)
  • Cancer Research

Cite this

A Bacterial Effector Co-opts Calmodulin to Target the Plant Microtubule Network. / Guo, Ming; Kim, Panya; Li, Guangyong; Elowsky, Christian G.; Alfano, James R.

In: Cell Host and Microbe, Vol. 19, No. 1, 13.01.2016, p. 67-78.

Research output: Contribution to journalArticle

Guo, Ming ; Kim, Panya ; Li, Guangyong ; Elowsky, Christian G. ; Alfano, James R. / A Bacterial Effector Co-opts Calmodulin to Target the Plant Microtubule Network. In: Cell Host and Microbe. 2016 ; Vol. 19, No. 1. pp. 67-78.
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