A bacterial E3 ubiquitin ligase targets a host protein kinase to disrupt plant immunity

Tracy R. Rosebrock, Lirong Zeng, Jennifer J. Brady, Robert B. Abramovitch, Fangming Xiao, Gregory B. Martin

Research output: Contribution to journalArticle

213 Citations (Scopus)

Abstract

Many bacterial pathogens of plants and animals use a type III secretion system to deliver diverse virulence-associated 'effector' proteins into the host cell. The mechanisms by which these effectors act are mostly unknown; however, they often promote disease by suppressing host immunity. One type III effector, AvrPtoB, expressed by the plant pathogen Pseudomonas syringae pv. tomato, has a carboxy-terminal domain that is an E3 ubiquitin ligase. Deletion of this domain allows an amino-terminal region of AvrPtoB (AvrPtoB1-387) to be detected by certain tomato varieties leading to immunity-associated programmed cell death. Here we show that a host kinase, Fen, physically interacts with AvrPtoB1-387and is responsible for activating the plant immune response. The AvrPtoB E3 ligase specifically ubiquitinates Fen and promotes its degradation in a proteasome-dependent manner. This degradation leads to disease susceptibility in Fen-expressing tomato lines. Various wild species of tomato were found to exhibit immunity in response to AvrPtoB1-387and not to full-length AvrPtoB. Thus, by acquiring an E3 ligase domain, AvrPtoB has thwarted a highly conserved host resistance mechanism.

Original languageEnglish (US)
Pages (from-to)370-374
Number of pages5
JournalNature
Volume448
Issue number7151
DOIs
StatePublished - Jul 19 2007

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Plant Immunity
Ubiquitin-Protein Ligases
Lycopersicon esculentum
Protein Kinases
Immunity
Pseudomonas syringae
Disease Susceptibility
Proteasome Endopeptidase Complex
Virulence
Cell Death
Phosphotransferases
Proteins

ASJC Scopus subject areas

  • General

Cite this

Rosebrock, T. R., Zeng, L., Brady, J. J., Abramovitch, R. B., Xiao, F., & Martin, G. B. (2007). A bacterial E3 ubiquitin ligase targets a host protein kinase to disrupt plant immunity. Nature, 448(7151), 370-374. https://doi.org/10.1038/nature05966

A bacterial E3 ubiquitin ligase targets a host protein kinase to disrupt plant immunity. / Rosebrock, Tracy R.; Zeng, Lirong; Brady, Jennifer J.; Abramovitch, Robert B.; Xiao, Fangming; Martin, Gregory B.

In: Nature, Vol. 448, No. 7151, 19.07.2007, p. 370-374.

Research output: Contribution to journalArticle

Rosebrock, TR, Zeng, L, Brady, JJ, Abramovitch, RB, Xiao, F & Martin, GB 2007, 'A bacterial E3 ubiquitin ligase targets a host protein kinase to disrupt plant immunity', Nature, vol. 448, no. 7151, pp. 370-374. https://doi.org/10.1038/nature05966
Rosebrock TR, Zeng L, Brady JJ, Abramovitch RB, Xiao F, Martin GB. A bacterial E3 ubiquitin ligase targets a host protein kinase to disrupt plant immunity. Nature. 2007 Jul 19;448(7151):370-374. https://doi.org/10.1038/nature05966
Rosebrock, Tracy R. ; Zeng, Lirong ; Brady, Jennifer J. ; Abramovitch, Robert B. ; Xiao, Fangming ; Martin, Gregory B. / A bacterial E3 ubiquitin ligase targets a host protein kinase to disrupt plant immunity. In: Nature. 2007 ; Vol. 448, No. 7151. pp. 370-374.
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