A 4-selenocysteine, 2-selenocysteine insertion sequence (SECIS) element methionine sulfoxide reductase from Metridium senile reveals a non-catalytic function of selenocysteines

Byung Cheon Lee, Alexey V. Lobanov, Stefano M. Marino, Alaattin Kaya, Javier Seravalli, Dolph L. Hatfield, Vadim N. Gladyshev

Research output: Contribution to journalArticle

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Abstract

Selenocysteine (Sec) residues occur in thiol oxidoreductase families, and functionally characterized selenoenzymes typically have a single Sec residue used directly for redox catalysis. However, how new Sec residues evolve and whether non-catalytic Sec residues exist in proteins is not known. Here, we computationally identified several genes with multiple Sec insertion sequence (SECIS) elements, one of which was a methionine-R-sulfoxide reductase (MsrB) homolog from Metridium senile that has four in-frame UGA codons and two nearly identical SECIS elements. One of the UGA codons corresponded to the conserved catalytic Sec or Cys in MsrBs, whereas the three other UGA codons evolved recently and had no homologs with Sec or Cys in these positions. Metabolic 75Se labeling showed that all four in-frame UGA codons supported Sec insertion and that both SECIS elements were functional and collaborated in Sec insertion at each UGA codon. Interestingly, recombinant M. senile MsrB bound iron, and further analyses suggested the possibility of binding an iron-sulfur cluster by the protein. These data show that Sec residues may appear transiently in genes containing SECIS elements and be adapted for non-catalytic functions.

Original languageEnglish (US)
Pages (from-to)18747-18755
Number of pages9
JournalJournal of Biological Chemistry
Volume286
Issue number21
DOIs
StatePublished - May 27 2011

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Selenocysteine
DNA Transposable Elements
Terminator Codon
methionine sulfoxide reductase
Methionine Sulfoxide Reductases
Iron
Genes
Iron-Sulfur Proteins
Catalysis
Sulfur
Sulfhydryl Compounds
Labeling

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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A 4-selenocysteine, 2-selenocysteine insertion sequence (SECIS) element methionine sulfoxide reductase from Metridium senile reveals a non-catalytic function of selenocysteines. / Lee, Byung Cheon; Lobanov, Alexey V.; Marino, Stefano M.; Kaya, Alaattin; Seravalli, Javier; Hatfield, Dolph L.; Gladyshev, Vadim N.

In: Journal of Biological Chemistry, Vol. 286, No. 21, 27.05.2011, p. 18747-18755.

Research output: Contribution to journalArticle

Lee, Byung Cheon ; Lobanov, Alexey V. ; Marino, Stefano M. ; Kaya, Alaattin ; Seravalli, Javier ; Hatfield, Dolph L. ; Gladyshev, Vadim N. / A 4-selenocysteine, 2-selenocysteine insertion sequence (SECIS) element methionine sulfoxide reductase from Metridium senile reveals a non-catalytic function of selenocysteines. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 21. pp. 18747-18755.
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