6;7 Chromosomal translocation in spontaneously arising rat immunocytomas

Evidence for c-myc breakpoint clustering and correlation between isotypic expression and the c-myc target

W. S. Pear, G. Wahlstrom, S. F. Nelson, H. Axelson, A. Szeles, F. Wiener, H. Bazin, G. Klein, Janos Sumegi

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Abstract

Our previous studies have shown that spontaneously arising immunocytomas in the LOU/Ws1 strain of rats contain a t(6;7) chromosomal translocation in all seven tumors studied (F. Wiener, M. Babonits, J. Spira, G. Klein, and H. Bazin, Int. J. Cancer 29:431-437, 1982). We have also shown that the c-myc is located on chromosome 7 (J. Sumegi, J. Spira, H. Bazin, J. Szpirer, G. Levan, and G. Klein, Nature (London) 306:497-499, 1983) and the immunoglobulin H cluster on chromosome 6 (W.S. Pear, G. Wahlstrom, J. Szpirer, G. Levan, G. Klein, and J. Sumegi, Immunogenetics 23:393-395, 1986). We now report a detailed cytogenetic and molecular analysis of nine additional rat immunocytomas. The t(6;7) chromosomal translocation is found in all tumors. Mapping of the c-myc breakpoints showed that in 10 of 14 tumors, the c-myc breakpoints are clustered in a 1.5-kilobase region upstream of exon 1. In contrast with sporadic Burkitt's lymphoma and mouse plasmacytoma, only 1 of 14 tumors contains the c-myc breakpoints in either exon 1 or intron 1. Analysis of the sequences juxtaposed to the c-myc show that immunoglobulin H switch regions are the targets in at least five tumors and that there is a strong correlation between the secreted immunoglobulin and the c-myc target. Unlike sporadic Burkitt's lymphoma and mouse plasmacytoma, at least two rat immunocytomas show recombination of the c-myc with sequences distinct from immunoglobulin switch regions.

Original languageEnglish (US)
Pages (from-to)441-451
Number of pages11
JournalMolecular and cellular biology
Volume8
Issue number1
DOIs
StatePublished - Jan 1 1988

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Genetic Translocation
Cluster Analysis
Immunoglobulin Switch Region
Neoplasms
Plasmacytoma
Burkitt Lymphoma
Immunoglobulins
Exons
Immunogenetics
Chromosomes, Human, Pair 6
Chromosomes, Human, Pair 7
Cytogenetic Analysis
Introns
Genetic Recombination
Sequence Analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

6;7 Chromosomal translocation in spontaneously arising rat immunocytomas : Evidence for c-myc breakpoint clustering and correlation between isotypic expression and the c-myc target. / Pear, W. S.; Wahlstrom, G.; Nelson, S. F.; Axelson, H.; Szeles, A.; Wiener, F.; Bazin, H.; Klein, G.; Sumegi, Janos.

In: Molecular and cellular biology, Vol. 8, No. 1, 01.01.1988, p. 441-451.

Research output: Contribution to journalArticle

Pear, W. S. ; Wahlstrom, G. ; Nelson, S. F. ; Axelson, H. ; Szeles, A. ; Wiener, F. ; Bazin, H. ; Klein, G. ; Sumegi, Janos. / 6;7 Chromosomal translocation in spontaneously arising rat immunocytomas : Evidence for c-myc breakpoint clustering and correlation between isotypic expression and the c-myc target. In: Molecular and cellular biology. 1988 ; Vol. 8, No. 1. pp. 441-451.
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