5′ terminal deletions in the genome of a coxsackievirus B2 strain occurred naturally in human heart

Nora M. Chapman, Kyung Soo Kim, Kristen M. Drescher, Kuniyuki Oka, Steven Tracy

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Enteroviruses can induce human myocarditis, which can be modeled in mice inoculated with group B coxsackieviruses (CVB) and in which CVB evolve to produce defective, terminally deleted genomes. The 5′ non-translated region (NTR) was enzymatically amplified from heart tissue of a fatal case of enterovirus-associated myocarditis in Japan in 2002. While no intact 5′ viral genomic termini were detected, 5′ terminal deletions ranged in size from 22 to 36 nucleotides. Sequence of the 5′ third of this viral genome is of a modern strain, closely related to CVB2 strains isolated in Japan in 2002. A CVB3 chimera containing the 5′ NTR with a 22 nt deletion produced progeny virus upon transfection of HeLa cells. When the 5′ 22 nucleotide deletion was repaired, the virus induced myocarditis in mice and replicated like wild type virus in murine heart cells. This is the first report of these naturally-occurring defective enteroviral genomes in human myocarditis.

Original languageEnglish (US)
Pages (from-to)480-491
Number of pages12
JournalVirology
Volume375
Issue number2
DOIs
StatePublished - Jun 5 2008

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Enterovirus
Myocarditis
Genome
Viruses
Japan
Nucleotides
Human Enterovirus B
Viral Genome
Human Genome
HeLa Cells
Transfection

Keywords

  • 5′ terminal deletion
  • Coxsackievirus
  • Defective enterovirus
  • Human myocarditis

ASJC Scopus subject areas

  • Virology

Cite this

5′ terminal deletions in the genome of a coxsackievirus B2 strain occurred naturally in human heart. / Chapman, Nora M.; Kim, Kyung Soo; Drescher, Kristen M.; Oka, Kuniyuki; Tracy, Steven.

In: Virology, Vol. 375, No. 2, 05.06.2008, p. 480-491.

Research output: Contribution to journalArticle

Chapman, Nora M. ; Kim, Kyung Soo ; Drescher, Kristen M. ; Oka, Kuniyuki ; Tracy, Steven. / 5′ terminal deletions in the genome of a coxsackievirus B2 strain occurred naturally in human heart. In: Virology. 2008 ; Vol. 375, No. 2. pp. 480-491.
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