5-Hydroxydecanoate is metabolised in mitochondria and creates a rate-limiting bottleneck for β-oxidation of fatty acids

Peter J. Hanley, Stefan Dröse, Ulrich Brandt, Rachel A. Lareau, Abir L. Banerjee, D. K. Srivastava, Leonard J. Banaszak, Joseph J. Barycki, Paul P. Van Veldhoven, Jürgen Daut

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Abstract

5-Hydroxydecanoate (5-HD) blocks pharmacological and ischaemic preconditioning, and has been postulated to be a specific inhibitor of mitochondrial ATP-sensitive K+ (KATP) channels. However, recent work has shown that 5-HD is activated to 5-hydroxydecanoyl-CoA (5-HD-CoA), which is a substrate for the first step of β-oxidation. We have now analysed the complete β-oxidation of 5-HD-CoA using specially synthesised (and purified) substrates and enzymes, as well as isolated rat liver and heart mitochondria, and compared it with the metabolism of the physiological substrate decanoyl-CoA. At the second step of β-oxidation, catalysed by enoyl-CoA hydratase, enzyme kinetics were similar using either decenoyl-CoA or 5-hydroxydecenoyl-CoA as substrate. The last two steps were investigated using l-3-hydroxyacyl-CoA dehydrogenase (HAD) coupled to 3-ketoacyl-CoA thiolase. Vmax for the metabolite of 5-HD (3,5-dihydroxydecanoyl-CoA) was fivefold slower than for the corresponding metabolite of decanoate (L-3-hydroxydecanoyl-CoA). The slower kinetics were not due to accumulation of D-3-hydroxyoctanoyl-CoA since this enantiomer did not inhibit HAD. Molecular modelling of HAD complexed with 3,5-dihydroxydecanoyl-CoA suggested that the 5-hydroxyl group could decrease HAD turnover rate by interacting with critical side chains. Consistent with the kinetic data, 5-hydroxydecanoyl-CoA alone acted as a weak substrate in isolated mitochondria, whereas addition of 100 μm 5-HD-CoA inhibited the metabolism of decanoyl-CoA or lauryl-carnitine. In conclusion, 5-HD is activated, transported into mitochondria and metabolised via β-oxidation, albeit with rate-limiting kinetics at the penultimate step. This creates a bottleneck for β-oxidation of fatty acids. The complex metabolic effects of 5-HD invalidate the use of 5-HD as a blocker of mitochondrial KATP channels in studies of preconditioning.

Original languageEnglish (US)
Pages (from-to)307-318
Number of pages12
JournalJournal of Physiology
Volume562
Issue number2
DOIs
StatePublished - Jan 15 2005

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Coenzyme A
Mitochondria
Fatty Acids
Oxidoreductases
5-hydroxydecanoic acid
Enoyl-CoA Hydratase
3-Hydroxyacyl-CoA Dehydrogenase
Acetyl-CoA C-Acyltransferase
Decanoates
Heart Mitochondria
KATP Channels
Ischemic Preconditioning
Carnitine
Liver Mitochondrion
Enzymes
Hydroxyl Radical
Adenosine Triphosphate
Pharmacology

ASJC Scopus subject areas

  • Physiology

Cite this

Hanley, P. J., Dröse, S., Brandt, U., Lareau, R. A., Banerjee, A. L., Srivastava, D. K., ... Daut, J. (2005). 5-Hydroxydecanoate is metabolised in mitochondria and creates a rate-limiting bottleneck for β-oxidation of fatty acids. Journal of Physiology, 562(2), 307-318. https://doi.org/10.1113/jphysiol.2004.073932

5-Hydroxydecanoate is metabolised in mitochondria and creates a rate-limiting bottleneck for β-oxidation of fatty acids. / Hanley, Peter J.; Dröse, Stefan; Brandt, Ulrich; Lareau, Rachel A.; Banerjee, Abir L.; Srivastava, D. K.; Banaszak, Leonard J.; Barycki, Joseph J.; Van Veldhoven, Paul P.; Daut, Jürgen.

In: Journal of Physiology, Vol. 562, No. 2, 15.01.2005, p. 307-318.

Research output: Contribution to journalArticle

Hanley, PJ, Dröse, S, Brandt, U, Lareau, RA, Banerjee, AL, Srivastava, DK, Banaszak, LJ, Barycki, JJ, Van Veldhoven, PP & Daut, J 2005, '5-Hydroxydecanoate is metabolised in mitochondria and creates a rate-limiting bottleneck for β-oxidation of fatty acids', Journal of Physiology, vol. 562, no. 2, pp. 307-318. https://doi.org/10.1113/jphysiol.2004.073932
Hanley, Peter J. ; Dröse, Stefan ; Brandt, Ulrich ; Lareau, Rachel A. ; Banerjee, Abir L. ; Srivastava, D. K. ; Banaszak, Leonard J. ; Barycki, Joseph J. ; Van Veldhoven, Paul P. ; Daut, Jürgen. / 5-Hydroxydecanoate is metabolised in mitochondria and creates a rate-limiting bottleneck for β-oxidation of fatty acids. In: Journal of Physiology. 2005 ; Vol. 562, No. 2. pp. 307-318.
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AU - Hanley, Peter J.

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AU - Brandt, Ulrich

AU - Lareau, Rachel A.

AU - Banerjee, Abir L.

AU - Srivastava, D. K.

AU - Banaszak, Leonard J.

AU - Barycki, Joseph J.

AU - Van Veldhoven, Paul P.

AU - Daut, Jürgen

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N2 - 5-Hydroxydecanoate (5-HD) blocks pharmacological and ischaemic preconditioning, and has been postulated to be a specific inhibitor of mitochondrial ATP-sensitive K+ (KATP) channels. However, recent work has shown that 5-HD is activated to 5-hydroxydecanoyl-CoA (5-HD-CoA), which is a substrate for the first step of β-oxidation. We have now analysed the complete β-oxidation of 5-HD-CoA using specially synthesised (and purified) substrates and enzymes, as well as isolated rat liver and heart mitochondria, and compared it with the metabolism of the physiological substrate decanoyl-CoA. At the second step of β-oxidation, catalysed by enoyl-CoA hydratase, enzyme kinetics were similar using either decenoyl-CoA or 5-hydroxydecenoyl-CoA as substrate. The last two steps were investigated using l-3-hydroxyacyl-CoA dehydrogenase (HAD) coupled to 3-ketoacyl-CoA thiolase. Vmax for the metabolite of 5-HD (3,5-dihydroxydecanoyl-CoA) was fivefold slower than for the corresponding metabolite of decanoate (L-3-hydroxydecanoyl-CoA). The slower kinetics were not due to accumulation of D-3-hydroxyoctanoyl-CoA since this enantiomer did not inhibit HAD. Molecular modelling of HAD complexed with 3,5-dihydroxydecanoyl-CoA suggested that the 5-hydroxyl group could decrease HAD turnover rate by interacting with critical side chains. Consistent with the kinetic data, 5-hydroxydecanoyl-CoA alone acted as a weak substrate in isolated mitochondria, whereas addition of 100 μm 5-HD-CoA inhibited the metabolism of decanoyl-CoA or lauryl-carnitine. In conclusion, 5-HD is activated, transported into mitochondria and metabolised via β-oxidation, albeit with rate-limiting kinetics at the penultimate step. This creates a bottleneck for β-oxidation of fatty acids. The complex metabolic effects of 5-HD invalidate the use of 5-HD as a blocker of mitochondrial KATP channels in studies of preconditioning.

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